急性盐度胁迫对日本黄姑鱼肌肉组织转录组的影响  

Transcriptome analysis of Nibea japonica under acute salinity stress

作　　者：孟玮 徐开达[1] 李振华[1] 史会来[1] 周永东[1] MENG Wei;XU Kaida;LI Zhenhua;SHI Huilai;ZHOU Yongdong(Marine Fishery Research Institute of Zhejiang Province,Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province,Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds,Ministry of Agriculture and Rural Affairs,Zhoushan 316021,China)

机构地区：[1]浙江省海洋水产研究所,浙江省海洋渔业资源可持续利用技术研究重点实验室,农业农村部重点渔场渔业资源科学观测实验站,浙江舟山316021

出　　处：《水产学报》2021年第5期649-660,共12页Journal of Fisheries of China

基　　金：国家重点研发计划(2017YFA0604904,2019YFD0901204);浙江省科技计划(2019C02056)。

摘　　要：盐度是影响鱼类各种生理活动的重要环境因素,为探讨急性盐度胁迫对经济海水鱼类日本黄姑鱼基因表达水平的影响,实验基于Illumina HiSeqTM 2500高通量测序平台,对不同盐度条件下日本黄姑鱼幼鱼肌肉组织进行转录组测序并开展生物信息学分析。结果显示,高盐组、低盐组和对照组分别获得46379598、36130844和38715820条Clean reads,总碱基数分别为5.27、4.87和5.53 GB。经组装得到91667条转录本,去冗余后拼接得到61601条Unigenes。与对照组相比,高盐组和低盐组分别有2230和1377个差异Unigene上调,1959和2447个差异Unigene下调,随机选取6条差异表达基因进行实时荧光定量PCR验证,结果与转录组测序一致。通过对显著差异表达基因进行筛选,在高盐组和低盐组中分别发现21和41个差异表达基因与离子通道相关,共有的离子通道相关基因为15个,其中14个基因表达趋势一致。而在高盐组和低盐组发现的离子转运体基因分别为6和10个。GO功能富集分析发现,差异基因显著富集在蛋白酶体、补体激活方面,KEGG通路富集分析发现,差异基因富集在绑定、催化活性、信号传导和分解代谢等方面。研究表明,日本黄姑鱼对急性盐度胁迫的适应可能是一个涉及多组织和多基因的复杂过程,盐度变化能够影响肌肉组织的离子通道、离子转运体,蛋白降解和免疫系统功能。研究结果为今后深入开展日本黄姑鱼盐度适应的调控机制及养殖育种研究提供了参考。Salinity is an important environmental factor that affects the physiological activities of fish.In order to investigate the changes of gene expression level in Nibea japonica,an economic marine fish treated by acute salinity stress,the transcriptomes of muscle tissues under different salinity conditions were sequenced based on the Illumina HiSeqTM 2500 high-throughput sequencing platform and the data were analyzed by bioinformatics tools in this study.A total of 46379598(5.27 Gb),36130844(4.87 Gb)and 38715820(5.53 Gb)clean reads were obtained in the high salinity group(HS),the low salinity group(LS)and the control group(C),respectively.91667 transcripts were assembled and 61601 unigenes were spliced after removing redundancy.Compared with the control group,2230 and 1377 differential expression genes(DEGs)were up-regulated,while 1959 and 2447 DEGs were down-regulated in the high salinity group and the low salinity group,respectively.Six DEGs were randomly selected for quantitative real-time PCR(qRT-PCR),and the results were consistent with the RNA-seq.21 and 41 ion channel genes were obtained from all significant DEGs in high salinity group and low salinity group,respectively.Fourteen of 15 shared genes showed the same trend.By contrast,6 and 10 ion transporter genes were acquired from the significant DEGs of two groups.The results of GO functional enrichment showed that the DEGs were significantly enriched in proteasome and complement activation,and KEGG pathway enrichment suggested that the DEGs were enriched in binding,catalytic activity,signal transduction and catabolism.The results indicated that the salinity adaptation of N.japonica is a complex process involving multiple tissues and genes.Besides the change of ion channels and ion transporters,the change of salinity also affects the protein degradation and immune system.The findings will provide important references for further study on the regulation mechanism of salinity adaptation and breeding of N.japonica.

关 键 词：日本黄姑鱼 急性盐度胁迫 肌肉 转录组测序 离子通道 离子转运体 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]