恩诺沙星及其代谢产物在珍珠龙胆石斑鱼(Epinephelus fuscoguttatus ♀× Epinephelus lanceolatus ♂)体内的代谢及消除规律  

Metabolism and elimination of enrofloxacin and its metabolites in(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)

作　　者：戴燕彬[1] DAI Yanbin(Fisheries Technical Extension Station of Zhangzhou,Zhangzhou 363000,China)

机构地区：[1]漳州市水产技术推广站,福建漳州363000

出　　处：《渔业研究》2021年第3期299-306,共8页Journal of Fisheries Research

基　　金：福建省海洋与渔业科技项目(闽财指〔2018〕850号).

摘　　要：本研究在连续给药的情况下,开展恩诺沙星及其代谢产物环丙沙星在珍珠龙胆石斑鱼体内的代谢残留情况研究,为科学合理使用恩诺沙星防治珍珠龙胆石斑鱼的细菌性疾病提供理论依据,也在水产养殖上为指导恩诺沙星科学用药提供借鉴。在28℃的水温条件下,以30 mg/(kg·bw)恩诺沙星对平均体重为(300±28)g的珍珠龙胆石斑鱼进行连续5 d口灌给药实验,用高效液相色谱-串联质谱法测定石斑鱼血浆、肌肉和肝脏中恩诺沙星及其代谢产物环丙沙星的残留含量。结果显示:恩诺沙星在血浆、肝脏和肌肉中很快达到最大值,达峰时间分别为第5天给药后3 h、第5天给药后3 h和第4天给药后3 h,其浓度分别为24.5 mg/L、13.0 mg/kg和8.20 mg/kg;恩诺沙星的代谢产物环丙沙星分别在第5天给药后3 h、第5天给药后3 h和第4天给药后3 h达到峰值,其浓度分别为0.276 mg/L、0.738 mg/kg和0.110 mg/kg,说明只有少量的恩诺沙星代谢成环丙沙星。根据本实验的数据,建议休药期不低于980度日。Under the condition of continuous administration,the present study investigated the metabolic residue of enrofloxacin and its metabolite ciprofloxacin in(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂),in order to provide theoretical basis for the scientific and reasonable use of enrofloxacin to prevent and treat the bacterial diseases of(E.fuscoguttatus♀×E.lanceolatus♂),and to provide reference for the guidance of enrofloxacin usage in aquaculture.(E.fuscoguttatus♀×E.lanceolatus♂)weighted(300±28)g were fed with 30 mg/(kg·bw)enrofloxacin by oral administration under the water temperature of 28℃,then the concentration of enrofloxacin and its metabolites ciprofloxacin in plasma,muscle and liver were determined by HPLC-MS.The results showed that enrofloxacin quickly reached the maximum in plasma,liver and muscle,while the peak time was 3 hours at the end of the fifth day,3 hours at the end of the fifth day and 3 hours at the end of the fourth day,respectively.The concentrations were 24.5 mg/L,13.0 mg/kg and 8.20 mg/kg,respectively.The peak time of ciprofloxacin in plasma,liver and muscle was 3 hours after the end of the fifth day,3 hours after the end of the fifth day and 3 hours after the end of the fourth day,and the concentrations were 0.276 mg/L,0.738 mg/kg and 0.110 mg/kg,respectively.Our results indicated that only a small amount of enrofloxacin was metabolized to ciprofloxacin,and it is suggested that the rest period should not be less than 980 degree-days.

关 键 词：恩诺沙星 环丙沙星 珍珠龙胆石斑鱼 代谢残留 休药期 

分 类 号：S948[农业科学—水产养殖]