北部湾卡盾藻(Chattonella spp.)种类鉴定  

Identification of strains of Chattonella spp. from the Beibu Gulf

作　　者：徐轶肖 何喜林 张腾 刘仁沿[3] 赵志娟 XU Yi-xiao;HE Xi-lin;ZHANG Teng;LIU Ren-yan;ZHAO Zhi-juan(Key Laboratory of Environment Change and Resources Use in Beibu Gulf,Ministry of Education,Nanning Normal University,Nanning 530001,China;Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation,Nanning Normal University,Nanning 530001,China;National Marine Environmental Monitoring Center,Dalian 116023,China;School of Environmental and Life Sciences,Nanning Normal university,Nanning 530001,China)

机构地区：[1]南宁师范大学,北部湾环境演变与资源利用教育部重点实验室,广西南宁530001 [2]南宁师范大学,广西地表过程与智能模拟重点实验室,广西南宁530001 [3]国家海洋环境监测中心,辽宁大连116023 [4]南宁师范大学环境与生命科学学院,广西南宁530001

出　　处：《海洋环境科学》2021年第3期352-360,共9页Marine Environmental Science

基　　金：国家自然科学基金项目(41976155,41506137);广西自然科学基金项目(2020GXNSFDA297001,2016GXNSFBA380037)。

摘　　要：2018年9月在北部湾海域分离得到3株卡盾藻,采用光学显微镜对其进行形态学初步鉴定,并通过核糖体大亚基LSU rDNA和ITS基因进行系统进化分析。结果表明,卡盾藻北部湾株符合海洋卡盾藻形态学典型特征:藻体黄褐色,细胞呈纺锤形,无黏液泡,尾部具尖端,细胞平均长(57.8±9.1)μm。系统发育树上北部湾卡盾藻分别以96%(LSU rDNA)和99%(ITS)的自展支持率与不同来源的海洋卡盾藻、古老卡盾藻、卵圆卡盾藻、小型卡盾藻聚在同一大分支上。通过遗传距离分析发现,北部湾3株卡盾藻与不同来源的海洋卡盾藻、古老卡盾藻、卵圆卡盾藻、小型卡盾藻亲缘关系较近,LSU和ITS遗传距离分别为0.0000~0.0016和0.0000~0.0235;而与盐生卡盾藻亲缘关系较远,遗传距离分别为0.0415~0.0490和0.0733~0.0750。因此,综合形态学和分子生物学结果可以确定北部湾3株卡盾藻为海洋卡盾藻/海洋卡盾藻的变种。研究结果可为我国卡盾藻藻华藻种库的构建和卡盾藻北部湾株生理生态学的研究提供基础数据。Three strains of Chattonella spp. were isolated from the Beibu Gulf in September 2018 and were identified using light microscopy and phylogenetic analysis based on LSU rDNA and ITS gene sequencing.Results show that the Chattonella spp. strains from the Beibu Gulf are yellowish-brown;the cells are spindleshaped with a pointed posterior end, and mucocysts are absent;the average cell length is(57.8±9.1) μm. These characteristics are consistent with the typical morphological characteristics of C. marina. In phylogenetic trees,the strains from the Beibu Gulf were clustered with C. marina, C. antiqua, C. ovata, and C. minima, with bootstrap value of 96% and 99% for LSU rDNA and ITS, respectively. Genetic distance analysis demonstrated that the three Chattonella spp. strains in the Beibu Gulf were closely related to C. marina, C. antiqua, C. ovata,and C. minima with genetic distances of 0.0000~0.0016 and 0.0000~0.0235, while being distantly related to C. subsalsa with genetic distances of 0.0415~0.0490 and 0.0733~0.0750 for LSU rDNA and ITS, respectively.Therefore, our comprehensive morphological and molecular biology methods suggested that the three strains of Chattonella spp. in the Beibu Gulf belonged to C. marina/C. marina var. antiqua/C. marina var. ovata. This study is helpful for Chattonella collection in the Beibu Gulf and will support further ecophysiological studies.

关 键 词：卡盾藻 系统发育树 遗传距离 藻华 北部湾 

分 类 号：Q178.53[生物学—水生生物学]