高压氧治疗改善大鼠颅脑损伤后神经功能障碍的效果及机制研究  

Effect of hyperbaric oxygen on improving the neurological dysfunction in rats with craniocerebral injury and its mechanism

作　　者：张杰[1] 师亚玲 黄卫东[1] 曹杰[1] Zhang Jie;Shi Yaling;Huang Weidong;Cao Jie(Department of Neurosurgery,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]陕西省人民医院神经外科,西安710068 [2]陕西省人民医院药学部,西安710068

出　　处：《中华航海医学与高气压医学杂志》2021年第2期224-228,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的观察高压氧治疗对于颅脑损伤大鼠神经功能障碍的治疗效果并探讨其相关机制。方法选取50只Sprague-Dawley大鼠,随机选取16只作为假手术对照组,剩余34只采用侧向液压打击(lateral fluid percussion,LFP)法进行颅脑损伤大鼠模型的构建。从建模成功的大鼠中按照随机数字表法选择16只作为脑损伤对照组(不进行高压氧治疗),另选16只颅脑损伤大鼠作为高压氧治疗组。高压氧治疗组于造模后6 h进行高压氧治疗,连续4周。通过水迷宫实验检测3组大鼠的空间记忆能力,并比较3组大鼠神经细胞凋亡数、胆碱乙酰转移酶(choline acetyltransferase,ChAT)阳性细胞数以及海马部位低氧诱导因子1α(HIF-1α)的表达情况。结果水迷宫实验中假手术对照组大鼠所用时间最短,高压氧治疗组次之,脑损伤对照组用时最长,差异均有统计学意义(P<0.05)。穿越站台区域的次数在假手术对照组最多,高压氧治疗组次之,脑损伤对照组最少,差异均有统计学意义(P<0.05)。空间探索实验中高压氧治疗组大鼠在站台附近的运动轨迹较密集,在目标象限的运动时长和运动距离较长。脑损伤对照组大鼠的神经细胞凋亡明显高于高压氧治疗组和假手术对照组,假手术对照组细胞凋亡最少。ChAT阳性细胞数在假手术对照组数量最多,高压氧治疗组次之,脑损伤对照组最少,组间比较差异有统计学意义(P<0.05);细胞平均截面积组间比较差异无统计学意义(P>0.05)。3组大鼠海马区HIF-a阳性细胞数在脑损伤对照组最多,高压氧治疗组次之,假手术对照组几乎无棕色细胞;3组大鼠大脑组织含水量及伊文氏蓝含量在假手术对照组最低,高压氧治疗组次之,脑损伤对照组最高,组间比较差异均有统计学意义(P<0.05)。结论高压氧治疗能够明显改善大鼠海马区神经细胞存活状况,减少细胞凋亡和HIF-1α表达,提高大鼠的学习与记忆能力,能够减轻局�Objective To observe the therapeutic effect of hyperbaric oxygen therapy on the neurological dysfunction in rats with craniocerebral injuries and to make a tentative study on its related mechanism.Methods Initially 50 Sprague-Dawley rats were prepared,among which 16 were randomly assigned to the sham operation control group.The remaining 34 were used to establish rat models with craniocerebral injury using lateral fluid percussion(LFP)method.The successful models were then randomly divided into the brain injury control group(n=16)and the hyperbaric oxygen therapy group(n=16).The latter group was treated with hyperbaric oxygen 6 hours after modeling.The spatial memory ability of all groups was tested in a water maze experiment.Furthermore,the apoptosis of rat nerve cells,the count of ChAT-positive cells,and the expression of HIF-1αin the hippocampus were compared among the three groups.Results In the water maze experiment,the rats in the sham operation control group used the shortest time,and the brain injury group was the slowest;the differences were statistically significant(P<0.05).In terms of the times of platform area crossing,the sham operation control group had the highest number of crossings,while the brain injury group made the lowest number;the differences were also statistically significant(P<0.05).In the spatial probe test,the rats in the hyperbaric oxygen treatment group had denser trajectories near the platform and longer exercise time and distance in the target quadrant.The apoptosis of nerve cells in the brain injury control group was significantly higher than those of the other two groups,and the sham operation control group had the least apoptosis;the differences among the groups were statistically significant(P<0.05).Regarding the count of ChAT-positive cells,the sham operation control group had the largest number,and the brain injury control group had the lowest one;the differences among the groups were statistically significant(P<0.05).The differences on the average cell cross-sectional areas

关 键 词：颅脑损伤 高压氧 神经功能 水迷宫 

分 类 号：R651.15[医药卫生—外科学]