电针预处理对术后认知功能障碍老龄小鼠海马氧化应激的影响  

Effect of electroacupuncture pretreatment on hippocampal oxidative stress in aged mice with postoperative cognitive dysfunction

作　　者：黎娜 洪素云[2] 李俊[2] 王改梅[3] 刘喆[2] 梁宜[2,3] LI Na;HONG Su-yun;LI Jun;WANG Gai-mei;LIU Zhe;LIANG Yi(Rehabilitation of Acupuncture-Moxibustion and Tuina,Ningbo Municipal Fenghua District Hospital of TCM,Ningbo 315500,Zhejiang Province,China;Laboratory Room of Neurobiology and Acupuncture Research,Third Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310053;Third Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005)

机构地区：[1]宁波市奉化区中医医院针灸推拿康复科,浙江宁波315500 [2]浙江中医药大学第三临床医学院浙江省针灸神经病学研究实验室,杭州310053 [3]浙江中医药大学附属第三医院,杭州310005

出　　处：《中国针灸》2021年第6期645-650,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81273822;国家重点基础研究发展计划(“973”计划)项目:2013CB531906。

摘　　要：目的:观察电针预处理对术后认知功能障碍(POCD)老龄小鼠海马氧化应激的影响,探讨电针预处理改善POCD老龄小鼠学习记忆能力的相关机制。方法:将72只健康雄性老龄小鼠随机分为空白组、模型组、药物组和电针组,每组18只,各组再分为1 d、3 d、7 d 3个亚组,每个亚组6只。电针组穴取“百会”“大椎”,予连续波(15 Hz,1 mA),持续干预30 min,每日1次,连续5 d;药物组予腹腔注射10%米诺环素(40 mg/kg),每日1次,连续5 d;空白组、模型组予腹腔注射等量的0.9%氯化钠溶液。除空白组外,其余各组小鼠均于干预结束后行肝叶部分切除术制备POCD模型。采用Morris水迷宫实验评估POCD老龄小鼠学习记忆能力,采用ELISA法测定各组小鼠海马组织活性氧(ROS)、丙二醛(MDA)含量,采用Western blot法检测各组小鼠海马组织超氧化物歧化酶1(SOD1)、超氧化物歧化酶2(SOD2)蛋白表达。结果:与空白组比较,模型组小鼠原平台象限停留时间百分比下降(P<0.01);药物组、电针组大鼠原平台象限停留时间百分比高于模型组(P<0.01)。与模型组比较,药物组各亚组及电针3 d组、7 d组ROS含量均降低(P<0.01,P<0.05),药物组、电针组MDA含量均降低(P<0.01,P<0.05),药物组各亚组及电针3 d组、7 d组海马SOD1、SOD2蛋白表达均增加(P<0.05,P<0.01);与药物组比较,电针1 d组ROS及MDA含量增加、SOD2蛋白表达降低(P<0.05),电针7 d组SOD1蛋白表达增加(P<0.05)。结论:电针预处理“大椎”“百会”可提高POCD老龄小鼠学习记忆能力,可能与其降低氧化应激反应水平、增强海马组织抗氧化能力有关。Objective To observe the effect of electroacupuncture(EA)pretreatment on hippocampal oxidative stress in aged mice with postoperative cognitive dysfunction(POCD)and explore the relevant mechanism of EA pretreatment on the improvement of learning and memory in POCD aged mice.Methods A total of 72 healthy male aged mice were randomized into a blank group,a model group,a medication group and an EA group,18 mice in each one.In each group,1-day,3-day and 7-day subgroups were divided separately,6 mice in each subgroup.In the EA group,"Baihui"(GV 20)and"Dazhui"(GV 14)were selected and stimulated with EA,using continuous wave(15 Hz,1 mA),continuously for 30 min,once a day,for 5 days consecutively.In the medication group,10%minocycline was injected intraperitoneally,40 mg/kg,once a day,consecutively for 5 days.In the blank and the control group,intraperitoneal injection of 0.9%sodium chloride solution was given with equal dosage.Except the blank group,at the end of intervention,partial hepatectomy was conducted to establish POCD model in the rest groups.Morris water maze test was adopted to evaluate the learning and memory ability of the aged mice.ELISA was used to determine the contents of reactive oxygen species(ROS)and malondialdehyde(MDA)in the hippocampal tissue.Western blot method was applied to detect the protein expressions of superoxide dismutase 1(SOD 1)and superoxide dismutase 2(SOD 2)in the hippocampal tissue.Results Compared with the blank group,the percentage of platform quadrant residence time was obviously reduced in the mice in the model group(P<0.01),and those in the medication group and the EA group were larger than the model group(P<0.01).Compared with the model group,the contents of ROS were reduced in each subgroup of the medication group and the 3-day subgroup and the 7-day subgroup of the EA group separately(P<0.01,P<0.05),MDA contents were reduced in the medication group and the EA group(P<0.01,P<0.05),the protein expressions of SOD 1 and SOD 2 were all increased in each subgroup of the medication

关 键 词：术后认知功能障碍 电针预处理 学习记忆 氧化应激 

分 类 号：R245.97[医药卫生—针灸推拿学]