保护地番茄根结线虫的病原鉴定及抗性材料筛选  

Pathogen Identification and Resistant Material Screening of Tomato Root-knot Nematode in Protected Field

作　　者：刘丹 尚春明 杨进[2] 张仙保 潘子旺 刘燕 郑于莉 LIU Dan;SHANG Chun-ming;YANG Jin(Baotou Institute of Agricultural Science,Baotou,Inner Mongolia 014013;Inner Mongolia Agricultural University,Baotou,Inner Mongolia 014013)

机构地区：[1]包头市农牧业科学研究院,内蒙古包头014013 [2]内蒙古农业大学职业技术学校,内蒙古包头014013

出　　处：《安徽农业科学》2021年第12期148-151,共4页Journal of Anhui Agricultural Sciences

基　　金：内蒙古自治区农牧业科学院青年创新基金资助(2018QN-JJN13);内蒙古重大专项(ZDZX201802)。

摘　　要：以根结线虫病样、番茄品种及组合为试材,采集包头市根结线虫暴发地一个根结线虫病样,进行形态学和分子生物学鉴定;利用PCR技术对24个番茄品种及组合进行Mi-1基因扩增筛选。结果表明,感染内蒙古包头市番茄的根结线虫为南方根结线虫;筛选出3个纯合抗根结线虫番茄组合。抗感品种及组合均产生750 bp的特异片段,纯合抗病基因的番茄组合存在TaqⅠ酶切位点,酶切后产生了570和160 bp特异性片段,为防治当地番茄根结线虫及培育抗病品种积累材料。Taking the root knot nematode samples and tomato varieties and combinations as test materials,a root knot nematode disease sample from the root knot nematode outbreak area in Baotou City was collected for morphological and molecular biological identification.The Mi-1 gene amplification and screening of 24 tomato varieties and combinations were carried out by PCR technology.The results showed that the root knot nematode infected tomato in Baotou City of Inner Mongolia was the southern root knot line,three homozygous tomato combinations with resistance to root knot nematode were screened out.The resistant varieties and combinations all produced 750 bp specific fragments.The Taq I restriction site existed in the homozygous resistant tomato combinations,and 570 and 160 bp specific fragments were produced after enzyme digestion,which were used to accumulate materials for the control of local tomato root knot nematode and the cultivation of disease resistant varieties.

关 键 词：番茄 根结线虫 分子生物学 

分 类 号：S433.1[农业科学—农业昆虫与害虫防治]