B亚型禽偏肺病毒对蛋鸡的致病性研究  

Pathogenicity of Avian Metapneumovirus Subtype Bon Layer Chicken

作　　者：包媛玲 何锡栋 于蒙蒙 刘鹏[1] 陶蓉蓉 肖美丽 王素艳 李欣翼 张卓[1] 刘爱晶[1] 张艳萍[1] 刘长军[1] 祁小乐[1] 王笑梅[1,3] 高玉龙 BAO Yuanling;HE Xidong;YU Mengmeng;LIU Peng;TAO Rongrong;XIAO Meili;WANG Suyan;LI Xinyi;ZHANG Zhuo;LIU Aijing;ZHANG Yanping;LIU Changjun;QI Xiaole;WANG Xiaomei;GAO Yulong(State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang 150069;Sunnzer Biotechnology Development Co.,Ltd.,Nanping,Fujian 354100;Jjiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou University,Yangzhou,Jiangsu 225009)

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江哈尔滨150069 [2]圣泽生物科技发展有限公司,福建南平354100 [3]扬州大学,江苏省动物重要疫病与人畜共患病协同创新中心,江苏扬州225009

出　　处：《中国家禽》2021年第6期25-30,共6页China Poultry

基　　金：现代农业产业技术体系专项资金(CARS-41-G15);黑龙江省自然科学基金(TD2019C003)

摘　　要：为研究B亚型禽偏肺病毒(aMPV/B)对蛋鸡的致病性,将aMPV/B LN16株经Vero细胞传代培养,通过点眼滴鼻的方式感染9周龄蛋鸡。感染后第2天,感染组蛋鸡开始出现流鼻涕,并伴有鼻痂等症状,发病率为92.3%。荧光定量PCR检测结果表明,感染组蛋鸡在感染后1~5 d排毒。感染后第4天,感染组蛋鸡的哈氏腺、气管、喉头及鼻甲骨内均有病毒分布。感染后7~21 d,感染组蛋鸡的aMPV抗体水平逐渐升高。组织病理观察结果表明,感染组蛋鸡的鼻甲骨、肺脏、气管均表现出不同程度的病理损伤,主要表现为炎性细胞浸润。研究表明,aMPV/B可以感染蛋鸡并引起发病。In order to study the pathogenicity of avian metapneumovirus subtype B(aMPV/B) to chickens, aMPV/B LN16 strain was cultured by Vero cells, and then infected 9-week-age laying hens through intraocular-nasal inoculation.After 2 days post infection(dpi), infected layers began to show symptoms of runny nose and nasal scabs, with an incidence of 92.3%. It was showed by RT-qPCR that the infected chickens exhibited viral shedding from 1 to 5 dpi.aMPV RNA was detected in Harderian gland, trachea, throat, and turbinate bones of infected layers at 4 dpi. The aMPV-specific antibody level increased gradually during 7 to 21 dpi. The turbinate, lung and trachea of the infected chickens showed pathological lesions to different degrees, mainly manifested as inflammatory cell infiltration. These results suggested that aMPV/B could infect layer chicken and cause disease.

关 键 词：B亚型禽偏肺病毒 蛋鸡 致病性 

分 类 号：S855.3[农业科学—临床兽医学]