地黄多糖对东莨菪碱诱导小鼠学习记忆能力障碍的改善作用及其机制  

Effects and mechanism of Rehmannia glutinosa polysaccharides on improving scopolamine-induced learning and memory impairment of mice

作　　者：马媛媛 姚晓英[1] 谈卓臣 廖茂行 MA Yuan-yuan;YAO Xiao-ying;TAN Zhuo-chen;LIAO Mao-xing(Shanghai Public Health Clinical Center,Shanghai 201508,China)

机构地区：[1]上海市公共卫生临床中心,上海201508

出　　处：《中成药》2021年第6期1462-1466,共5页Chinese Traditional Patent Medicine

摘　　要：目的研究地黄多糖对东莨菪碱诱导小鼠学习记忆能力障碍的改善作用及机制。方法小鼠随机分为正常组,模型组、多奈哌齐组(3 mg/kg)及地黄多糖高、低剂量组(30、15 mg/kg),灌胃给药28 d,除正常组外其余各组在给药第24天时腹腔注射3 mg/kg氢溴酸东莨菪碱,连续5 d。检测学习记忆能力,ChAT(乙酰胆碱转移酶)、AChE(乙酰胆碱酯酶)、SOD(超氧化物歧化酶)、CAT(过氧化氢酶)活性,MDA(丙二醛)、TNF-α(肿瘤坏死因子-α)、IL-1β(白细胞介素-1β)水平及NF-κB p65(兔源核因子κB p65)、IκB-α(NF-κB抑制蛋白α)蛋白表达。结果与模型组比较,地黄多糖高剂量组从第1天开始、低剂量组从第2天开始逃避潜伏期及各剂量组小鼠首次穿越平台时间降低(P<0.05,P<0.01),而地黄多糖各剂量组在第Ⅲ象限路程百分比、第Ⅲ象限停留时间、穿越平台次数增加(P<0.01)。与模型组比较,地黄多糖高、低剂量组小鼠海马组织ChAT、SOD、CAT活性及IκB-α表达增加(P<0.05,P<0.01),而AChE活性、MDA、TNF-α、IL-1β水平及NF-κB p65表达减少(P<0.01)。结论地黄多糖可改善东莨菪碱诱导的小鼠学习记忆能力障碍,该作用与调节胆碱能神经系统功能、抗氧化应激及抑制炎症反应有关。AIM To study the effects and mechanism of polysaccharides from Rehmannia glutinosa Libosch(RGLPs)on scopolamine-induced learning and memory impairment in mice.METHODS Mice were randomly divided into normal group,model group,donepezil group(3 mg/kg)and high-dose and low-dose RGLPs groups(30 and 15 mg/kg)for 28-day gavage administration of corresponding drugs.All the groups,except the normal group,started 5 consecutive days’intraperitoneal injection of scopolamine hydrobromide(3 mg/kg)from the 24th day of administration.The mice had the assessment of ability of learning and memory,ChAT,AChE,SOD and CAT activities,and MDA,TNF-αand IL-1βlevels,and NF-κB p65 and IκB-αprotein expressions.RESULTS Compared with the model group,RGLPs groups shared quicker speed at the first time when crossing the platform(P<0.01);and shorter escape latency period as the high-dose group reported it on day 1 and low-dose group on day 2(P<0.05);moreⅢquadrant distance percentage,Ⅲquadrant residence time and frequency in crossing the platform(P<0.01).Still in contrast to the model group,RGLPs groups demonstrated increased activities of ChAT,SOD and CAT,and protein expression of IκB-αin the hippocampu of mice(P<0.05 or P<0.01),and decreased AChE activity,MDA,TNF-αand IL-1βlevels,and NF-κB p65 protein expression(P<0.01).CONCLUSION RGLPs can improve scopolamine-induced learning and memory impairment in mice,which is related to their impact in regulating cholinergic nervous system function,anti-oxidative stress and inhibition of inflammatory response.

关 键 词：地黄多糖 胆碱能神经系统 氧化应激 炎症反应 NF-ΚB信号通路 

分 类 号：R285.5[医药卫生—中药学]