超高效液相色谱质谱联用法测定血液中伊维菌素和丙硫苯咪唑的残留量  

Determination of blood residues of ivermectin and albendazole by ultra performance liquid chromatography-mass spectrometry

作　　者：方雅莉 FANG Ya-li(Research Service Management Center,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学科研服务管理中心,广东东莞523808

出　　处：《广东医科大学学报》2021年第3期264-266,共3页Journal of Guangdong Medical University

摘　　要：目的了解超高效液相色谱质谱联用法测定血液中伊维菌素和丙硫苯咪唑的残留量的效果。方法血样用乙腈沉淀蛋白过滤提取伊维菌素和丙硫苯咪唑,以乙腈和5 mmol/L乙酸铵+0.1%甲酸水溶液为流动相,经C18色谱柱洗脱分离,采用电喷雾离子源,多反应监测正离子模式进行分析。结果在10~5000μg/L添加范围内,质谱响应强度与其质量浓度呈良好的线性关系。丙硫苯咪唑的检出限为5μg/L,定量检出限为5μg/L;伊维菌素检出限为0.5μg/L,定量检出限为1μg/L。结论该方法处前处理简单,准确度高,精密度好,可以为人畜中毒提供快速安全检测方法。Objective To investigate the feasibility of ultra performance liquid chromatography-mass spectrometry(UPLC-MS) in blood residues of ivermectin and albendazole. Methods Blood ivermectin and albendazole were extracted using acetonitrile precipitated protein. Acetonitrile, 5 mmol/L ammonium acetate and 0.1% formic acid aqueous solution used as mobile phase, the samples were eluted by C18 column and then analyzed by UPLC/electrospray ionization tandem MS. Results There was a good linear relationship between response intensity and mass concentration at the range of 10-5 000 μg/L. The detection limit and limit of quantization were 5 μg/L and 5 μg/L for albendazole, and 0.5 μg/L and 1 μg/L for ivermectin, respectively. Conclusion UPLC-MS is simple, accurate and precise for detecting blood residues of ivermectin and albendazole, which can provide a rapid and safe detection method for human and animal poisoning.

关 键 词：丙硫苯咪唑 伊维菌素 超高效液相色谱 质谱分析 

分 类 号：O61[理学—无机化学]