基于贝叶斯网络的基因与职业紧张交互作用对石油工人高血压影响  

Bayesian network-based analysis on the influence of interaction between genes and occupational stress on hypertension in oil workers

作　　者：安恒庆[1,2] 徐蕾 张园月 陶宁 AN Heng-qing;XU Lei;ZHANG Yuan-yue;TAO Ning(Postdoctoral Station of Public Health and Preventive Medicine,Xinjiang Medical University,Urumqi,Xinjiang 830011,China)

机构地区：[1]新疆医科大学公共卫生与预防医学博士后流动站,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院,新疆乌鲁木齐830011 [3]新疆医科大学公共卫生学院流行病与卫生统计学教研室,新疆乌鲁木齐830011 [4]新疆医科大学临床博士后流动站,新疆乌鲁木齐830011

出　　处：《中国职业医学》2021年第1期39-45,共7页China Occupational Medicine

基　　金：新疆维吾尔自治区自然科学基金(2020D01C158);中国博士后第64批面上资助西部地区人才项目(2018M643826XB);新疆维吾尔自治区高校科研计划项目(XJEDU2018Y029)。

摘　　要：目的分析贝叶斯网络(BN)构建的基因-基因、基因-职业紧张交互作用对石油工人高血压的影响。方法采用典型抽样方法,选择1 233名新疆克拉玛依油田公司的石油工人为研究对象,测量其血压,采用聚合酶链式反应限制性片段长度多态性技术检测α-内收蛋白(ADD1)、β_(2)-肾上腺素受体(β_(2)-AR)基因的半核苷酸多态性(SNP),采用《付出-回报失衡问卷》评价其付出-回报失衡(ERI)模式职业紧张,采用BN进行基因-基因、基因-职业紧张交互作用分析。结果研究对象ADD1 rs17833172位点的AA、AG、GG基因型分布和等位基因A、G基因频率分布比较,差异均有统计学意义(P值均<0.05);Gly460Trp rs4961位点中GG、GT、TT基因型分布比较,差异无统计学意义(P>0.05),等位基因G、T基因频率分布比较,差异有统计学意义(P<0.05);β_(2)-AR-1023G/A rs2053044、+252G/A rs1042717位点中AA、AG、GG基因型分布和等位基因A、G基因频率分布比较,差异均无统计学意义(P值均>0.05)。研究对象高血压、ERI模式职业紧张检出率分别为25.5%(315/1 233)和47.4%(585/1 233)。控制性别、文化程度、婚姻状况、吸烟、饮酒、体质量指数等混杂因素后,职业紧张组人群罹患高血压的风险高于非职业紧张组(比值比为1.33,95%可信区间为1.01~1.74,P<0.05)。BN构建的有向无环图显示:ADD1基因rs17833172、Gly460Trp rs4961位点均直接指向高血压,β_(2)-AR+252G/A rs1042717位点通过指向ADD1 rs17833172和Gly460Trp rs4961位点间接指向高血压;但职业紧张与ADD1、β_(2)-AR基因位点间的作用不指向高血压。结论石油工人罹患高血压与ERI模式职业紧张、ADD1基因rs17833172、Gly460Trp rs4961位点多态性均有关联;β_(2)-AR+252G/A rs1042717位点与ADD1Gly460Trp rs4961、rs17833172位点的基因-基因交互作用与石油工人罹患高血压有关联;未发现ADD1和β2-AR的4个SNP位点与ERI模式职业紧张之间的交互作用对石油工人罹患Objective To analyze the effect of gene-gene and gene-occupational stress interaction on hypertension in oil workers by Bayesian network(BN) analysis. Methods A total of 1 233 oil workers from Xinjiang Karamay Oilfield Company were selected as research subjects by the typical sampling method. The blood pressure was measured. The polymerase chain reaction-restriction fragment length polymorphism was used to evaluate the single nucleotide polymorphism(SNP) in α-adductin(ADD1) and β_(2)-adrenal receptor(β_(2)-AR) genes. The Effort-Reward Imbalance(ERI) Questionnaire was used to evaluate the ERI model occupational stress of oil workers. BN was used to analyze gene-gene and gene-occupational stress interactions. Results The detection rate of hypertension and ERI model occupational stress of these oil workers were 25.5%(315/1 233) and 47.4%(585/1 233) respectively. The AA, AG, and GG genotype and the frequency distribution of allele A and G at ADD1 rs17833172 were statistically different in these two groups(all P<0.05). There was no statistical significance in genotypes of GG, GT and TT at Gly460Trp rs4961(P>0.05);but the difference of the frequency of G and T alleles was statistically significant(P<0.05). There was no statistical significance in genotype distribution of AA, AG and GG, and frequency distribution of A and G in β_(2)-AR-1023 G/A rs2053044 and+252 G/A rs1042717(all P>0.05). After controlling the confounding factors including gender, education level, marital status, smoking, alcohol drinking and body mass index, the occupational stress group had a higher risk of hypertension than the no-occupational stress group(P<0.05, the odds ratio was 1.33, the 95% confidence interval was 1.01-1.74). The directed acyclic graph constructed by BN showed that the rs17833172 and Gly460Trp rs4961 sites of the ADD1 gene was directly associated with hypertension, and the β_(2)-AR+252 G/A rs1042717 site was indirectly associated with hypertension because of the ADD1 rs17833172 and Gly460Trp rs4961 sites. The interaction b

关 键 词：职业紧张 基因 高血压 贝叶斯网络 交互作用 

分 类 号：R135[医药卫生—劳动卫生]