水稻雄性不育系的胚囊育性及其对异交结实的影响  

Studies of embryo sacs fertility and its effect on outcrossing seed setting rate of male sterile lines in rice

作　　者：罗方雄 黄叔贤 郭海滨[3] 陈雄辉[2] 梁铁军 张泽民[2] 彭海峰[1] LUO Fangxiong;HUANG Shuxian;GUO Haibin;CHEN Xionghui;LIANG Tiejun;ZHANG Zemin;PENG Haifeng(College of Life Sciences,South China Agricultural University,Guangzhou,Guangdong 510642,China;College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642,China;Center of Experimental Teaching for Common Basic Courses,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学生命科学学院,广东广州510642 [2]华南农业大学农学院,广东广州510642 [3]华南农业大学公共基础实验教学中心,广东广州510642

出　　处：《湖南农业大学学报（自然科学版）》2021年第3期247-253,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：广东省自然科学基金项目(05006667)。

摘　　要：以3个光温敏核不育系W7415S、安农S、培矮64S和4个质核互作雄性不育系珍汕97A(夜公胞质)、珍汕97A(野败胞质)、华农A(夜公胞质)和华农A(野败胞质)为材料,通过观察各不育系成熟胚囊结构,并对异交结实率进行调查,以明确水稻雄性不育系异交结实率是否与胚囊育性有关。结果表明:不同水稻雄性不育系的胚囊育性与异交结实率呈显著正相关;对于光温敏核不育系,胚囊育性受不育系遗传、环境以及两者互作的影响,不育期的胚囊育性比可育期低,可育期的胚囊育性接近早季质核互作不育系;对于质核互作不育系,胚囊育性主要受环境的影响,早季比晚季的胚囊育性低;光温敏核不育系的胚囊对环境的敏感度高于质核互作不育系,尤其是W7415S对环境的影响较为敏感。水稻雄性不育系的胚囊育性是影响其异交结实的重要因素,在选育不育系时需考虑胚囊育性及其对环境的敏感度。In this study,three photo-thermo-sensitive genic male sterile(PTGMS)lines and four cytoplasmic-nuclear male sterile(CMS)lines were used as materials to investigate the embryo sacs fertility and outcrossing seed setting rate.Three PTGMS lines were W7415 S,AnnongS and Peiai 64 S.Four CMS lines were Zhenshan 97 A with wild abortion male sterile cytoplasm,Zhenshan 97 A with Yegong male sterile cytoplasm,HuanongA with wild abortion male sterile cytoplasm and HuanongA with Yegong male sterile cytoplasm.The results showed that the embryo sac fertility of the male sterile lines was positively correlated with their outcrossing seed setting rate.For the PTGMS lines,the embryo sac fertility was affected by the heredity,environment and their interaction,and the embryo sac fertility at sterile period was lower than that at fertile period.The embryo sac fertility of the PTGMS lines at fertile period was close to that of the CMS lines in the early season.For the CMS lines,the embryo sac fertility was mainly affected by the environment,and the embryo sac fertility in the early season was lower than that in the late season.Furthermore,the embryo sac of PTGMS lines was more sensitive to the environment than that of CMS lines,especially W7415 S.In conclusion,the embryo sac fertility is an important factor affecting the outcrossing seed setting of male sterile lines in rice,and embryo sacs fertility is affected by photoperiod and temperature in some PTGMS lines.The effect of embryo sac fertility should be considered in the breeding of male sterile lines in rice.

关 键 词：水稻 光温敏核不育系 质核互作不育系 胚囊育性 异交结实率 

分 类 号：S511.01[农业科学—作物学]