基于PCR的植物病毒检测技术研究进展  

Progress in PCR-based techniques for detection of plant viruses

作　　者：王森 陈东亮[2,3] 黄丛林 潘晓飞[4] 李彦慧 Wang Sen;Chen Dongliang;Huang Conglin;Pan Xiaofei;Li Yanhui(College of Landscape and Tourism,Hebei Agriculture Univer-sity,Baoding 071000,China;Beijing Agricultural Biotechnology Research Center,Beijing Academy of A-griculture and Forestry Sciences/Beijing Key Laboratory of Agricultural Gene Resources and Biotechnol-ogy;Beijing Functional Flower Engineering Technology Research Center;Natural Resources and Plan-ning Bureau of Daming County)

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000 [2]北京市农林科学院北京农业生物技术研究中心/农业基因资源与生物技术北京市重点实验室 [3]北京市功能花卉工程技术研究中心 [4]大名县自然资源和规划局

出　　处：《植物检疫》2021年第3期1-8,共8页Plant Quarantine

基　　金：北京市农林科学院科研创新能力建设专项(KJCX20200106,KJCX20200211)。

摘　　要：植物病毒是引起植物重大病害的病原之一。植物病毒病危害大、防治难度高,一旦感染病毒终生携带,而早期的检测成为病毒预防及治理的先决条件。基于基因扩增技术的病毒检测方法,由于检测迅速、敏感性高、成本低等优点而被广泛应用于各种病毒的检测。本文对常用的基于PCR的病毒检测方法进行了综述,并对其优缺点进行了比较,以期为选择合适的植物病毒检测方法提供参考。Plant virus is a kind of pathogen causing harmful diseases in plants.Because of great loss,hard to control and persistent infection,early diagnosis becomes the precondition of virus prevention and management.Virus detection method based on gene amplification is widely used in many kinds of virus detection due to its advantages of rapid detection,high sensitivity and low cost.In this paper,the commonly used virus detection methods based on PCR techniques are reviewed,and the advantages and disadvantages of them are discussed,so as to provide a reference for the method selection in detection of plant virus.

关 键 词：植物病毒 检测 聚合酶链式反应 

分 类 号：S4[农业科学—植物保护]