Wnt/β-catenin信号转导通路在瘢痕疙瘩形成中的作用机制研究  

Study on acting mechanisms of Wnt/β-catenin signaling transduction pathway in keloid formation

作　　者：费猛 麻艺群 崔倩[1] 李改赢 郭一俨 汤諹[1] FEI Meng;MA Yiqun;CUI Qian;LI Gaiying;GUO Yiyan;TANG Yang(Department of Dermatological,The First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院皮肤科,云南昆明650032

出　　处：《云南医药》2021年第3期207-211,共5页Medicine and Pharmacy of Yunnan

基　　金：云南省科技厅面上项目(2017FE468(-049));云南省科技厅应用基础研究(2011FB174)。

摘　　要：目的了解Wnt/β-catenin信号转导通路在瘢痕疙瘩原代成纤维细胞中关键蛋白和m RNA水平,探讨其在瘢痕疙瘩形机制中的作用。方法采用组织块培养法进行原代成纤维细胞培养,通过Western Blot和RT-PCR法分别检测正常皮肤以及瘢痕疙瘩成纤维细胞中Wnt/β-catenin信号转导通路中相关信号分子Wnt5a、β-catenin、GSK-3β、p-GSK-3β蛋白和Wnt5a、β-catenin、GSK-3βm RNA水平,比较两者之间差异。结果与正常皮肤相比,瘢痕疙瘩成纤维细胞中Wnt5a、β-catenin、p-GSK-3β蛋白及Wnt5a、β-catenin m RAN水平增高(P<0.05),GSK-3β蛋白及m RNA表达水平无明显变化。结论在瘢痕疙瘩形成过程中,Wnt5a、β-catenin m RAN上调并导致Wnt5a、β-catenin蛋白水平增高以及GSK-3β蛋白磷酸化导致p-GSK-3β增高可能在瘢痕疙瘩的形成机制中起着重要作用。Objective To investigate the key protein and m RNA levels of Wnt/β-Catenin signal transduction pathway in the primary keloid fibroblasts,and their roles in formation mechanism of keloid.Methods Primary fibroblasts were cultured by tissue culture.The protein and m RNA levels of Wnt5 a,β-Catenin,GSK-3β,p-GSK-3β of Wnt/β-Catenin signal transduction pathway in normal skin and the keloid fibroblasts were respectively detected by Western blot and RT-PCR.Data were analyzed by SPSS18.0 software.Results Compared with normal skin,the protein levels of Wnt5 a,β-Catenin,p-GSK-3β and m RNA of Wnt5 a,β-Catenin in keloid fibroblasts were increased(P<0.05),but the expression levels of GSK-3βprotein and m RNA did not significantly change.Conclusions In the process of keloid formation,both m RNA and protein levels of Wnt5 a and β-Catenin up-regulated.Phosphorylation of GSK-3β protein led to the upregulation of p-GSK-3β,which may play an important role in the formation of keloid.

关 键 词：瘢痕疙瘩 GSK-3Β WNT/Β-CATENIN信号转导通路 

分 类 号：R751[医药卫生—皮肤病学与性病学]