姜黄素催化生姜提取物的光化学反应及抗菌活性研究  

Studies on Curcumin-catalyzed Photochemical Reaction of Zingiber officinale Extract and Its Antimicrobial Activity

作　　者：黎晓菊 张晓梅 庹呈杰 张科涛 秦杰琛 张冰 黄之镨 赵庆 LI Xiaoju;ZHANG Xiaomei;TUO Chengjie;ZHANG Ketao;QIN Jiechen;ZHANG Bing;HUANG Zhipu;ZHAO Qing(Yunnan University of Medicine,Kunming 650500,China;Kunming Medical University,Kunming 650500,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]昆明医科大学,云南昆明650500

出　　处：《云南中医学院学报》2021年第1期24-29,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81460533,21662048,81060262);云南省科技厅-云南中医学院应用基础研究联合专项基金[2017FF116(-011)]。

摘　　要：目的 研究生姜提取物在姜黄素催化下的光化学反应,以及化学成分与抗菌活性的变化。方法采用纸片扩散法,测试光照后的生姜提取液,加入姜黄素、未光照的生姜提取液,加姜黄素并经过光照的生姜提取液,对23种病原菌的抗菌活性。采用薄层色谱法与高效液相色谱法检测加入姜黄素的生姜提取液光照后化学成分的变化。结果加入姜黄素的生姜提取液经光照处理后,对大多数菌株的抗菌活性明显增强,尤其对耐甲氧西林金黄色葡萄球菌(MRSA)及耻垢分枝杆菌的抗菌活性增强显著。薄层色谱和高效液相色谱显示加入姜黄素的生姜提取液在光照后,化学成分发生了明显变化。结论在姜黄素的催化下,生姜的化学成分在光照时发生光化学反应,生成了抗菌活性更高的产物。Objective Photochemical reaction of Zingiber officinale extract catalyzed by curcumin,and antimicrobial activities of the extracts before and after illumination were studied.Methods Disk diffusion test was applied to test the antimicrobial activities of the extract without curcumin after illumination,the extract containing curcumin after illumination,and that containing curcumin without illumination,against 23 kinds of microbial pathogens.TLC and HPLC were applied to detect the changes of the components in the Zingiber officinale extract after illumination,with or without curcumin.Results The antimicrobial activity of the illuminated extract containing curcumin increased significantly,especially the activities against MRSA and Mycobacterium smegmatis increased drastically.TLC and HPLC revealed that the components in Zingiber officinale extract containing curcumin changed significantly after illumination.Conclusion Under the catalysis of curcumin,some compounds in Zingiber officinale underwent photochemical reaction after illumination.As a result,products with much higher antimicrobial activity were generated.

关 键 词：生姜 姜黄素 光化学反应 抗菌活性 

分 类 号：R285[医药卫生—中药学]