我国东部沿海地区江河入海口水体中致病性弧菌分布特征  

Distribution characteristics of pathogenic Vibrio in estuary of eastern coastal rivers in China

作　　者：秦政 胡锦瑞 吴科明[3] 岑迪[4] 田云龙[5] 王春东[6] 李叶青[3] 杨铭锋 李燕 卢俊荣[6] 阚飙[1] 周海健[1] 包名家 崔志刚[1] QIN Zheng;HU Jin-rui;WU Ke-ming;CEN Di;TIAN Yun-long;WANG Chun-dong;LI Ye-qing;YANG Ming-feng;LI Yan;LU Jun-rong;KAN Biao;ZHOU Hai-jian;BAO Ming-jia;CUI Zhi-gang(National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;School of Public Health,Jiamusi University,Jiamusi,Heilongjiang 154007,China;Fangchenggang Center for Disease Control and Prevention,Fangchenggang,Guangxi 538001,China;Cixi Center for Disease Control and Prevention,Cixi,Zhejiang 315300,China;Yantai Center for Disease Control and Prevention,Yantai,Shandong 264003,China;Cangzhou Center for Disease Control and Prevention,Cangzhou,Hebei 061000,China;Jiamusi Center for Disease Control and Prevention,Jiamusi,Heilongjiang 154000,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206 [2]佳木斯大学公共卫生学院,黑龙江佳木斯154007 [3]防城港市疾病预防控制中心,广西防城港538001 [4]慈溪市疾病预防控制中心,浙江慈溪315300 [5]烟台市疾病预防控制中心,山东烟台264003 [6]沧州市疾病预防控制中心,河北沧州061000 [7]佳木斯市疾病预防控制中心,黑龙江佳木斯154000

出　　处：《中国热带医学》2021年第6期501-504,共4页China Tropical Medicine

基　　金：“十三五”国家科技重大专项(No.2018ZX10201002-005)。

摘　　要：目的分析我国东部沿海江河入海口水域的弧菌分布以及不同季节弧菌种类的差异,为致病性弧菌环境检测策略的制定提供数据支持。方法分别在2019年8月和2020年1月,在我国濒临渤海、黄海、东海、南海的4个监测地市分别挑选2条河流,在入海口采集水样,进行弧菌分离培养,每份水样挑选30个疑似弧菌菌落,通过微生物质谱仪进行鉴定。分析不同海域、不同季节的弧菌分布特征。结果在山东省烟台市、浙江省慈溪市、河北省沧州市和广西自治区防城港市4个监测地市进行水样采集,共采集水样32份,经分离纯化培养,共得到菌株960株,通过基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization time-of-flight mass spectrometry,MALDI-TOF MS)技术鉴定,最终鉴定出弧菌562株,其中副溶血弧菌211株(占22.0%)、霍乱弧菌172株(占17.9%)、溶藻弧菌124株(占12.9%),其余弧菌8种共55株(占5.7%)。4个海域弧菌优势弧菌分布存在显著差异,其中黄海(山东烟台)、东海(浙江慈溪)以霍乱弧菌为主,渤海(河北沧州)以副溶血弧菌和溶藻弧菌为主,南海(广西防城港)以副溶血弧菌为主。夏季分离弧菌优势菌株为霍乱弧菌,共148株,占该季节全部分离弧菌的30.8%;冬季分离弧菌优势菌株为副溶血弧菌,共65株,占该季节全部分离弧菌的13.5%,夏季、冬季分离的优势弧菌分布差异有统计学意义。结论我国东部沿海江河入海口水域的弧菌分布存在海域和季节性差异。Objective To analyze the distribution of Vibrio in China's coastal rivers and the differences of Vibrio species in different seasons,so as to provide data support for the formulation of environmental detection strategy of pathogenic Vibrio.Methods In August 2019 and January 2020,2 rivers were selected from 4 monitored cities in Bohai Sea,Yellow Sea,East China Sea and South China Sea,respectively.Water samples were collected at the estuary for isolation and culture of Vibrio.30 suspected Vibrio colonies were selected from each water sample and identified by microbial mass spectrometer.The distribution characteristics of Vibrio in different sea areas and different seasons were analyzed.Results In Yantai city of shandong province,Cixi city of Zhejiang province,Cangzhou city of Hebei province,and Fangchenggang city of Guangxi autonomous region,the 32 water samples collected from the four representative cities,960 strains got from the separation and purification,by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)technical appraisal,in the end,562 strains of Vibrio were identified,including 211 strains of Vibrio parahaemolyticus(22.0%),172 strains of Vibrio cholerae(17.9%),124 strains of Vibrio alginolyticus(12.9%),and 55 strains(5.7%)of the other 8 species.There were significant differences in the distribution of dominant Vibrio in the four sea areas.Vibrio cholerae was dominant in the Yellow Sea(Yantai,Shandong)and the East China Sea(cixi,Zhejiang),Vibrio parahaemolyticus and Vibrio alginolyticus were dominant in the Bohai Sea(Cangzhou,Hebei),and Vibrio parahaemolyticus was dominant in the South China Sea(Fangchenggang,Guangxi).The dominant strain of Vibrio isolated in summer was Vibrio cholerae(148 strains),accounting for 30.8% of all Vibrio isolated in this season.The dominant strain of Vibrioisolated in winter was Vibrio alginolyticus,a total of 65 strains,accounting for 13.5%of the total Vibrio isolated in the season.There was significant difference in the distribution of

关 键 词：弧菌 致病性弧菌 江河入海口 基质辅助激光解吸电离飞行时间质谱 

分 类 号：R378.3[医药卫生—病原生物学]