大豆GmNramp2a基因克隆及亚细胞定位和组织表达分析  

Cloning,Subcellular Localization and Tissue Expression Analysis of GmNramp2a Gene in Glycine max

作　　者：刘颖[1] 李小豪 陈经烨 陈晶晶[2] 薛迎斌 Liu Ying;Li Xiaohao;Chen Jingye;Chen Jingjing;Xue Yingbin(College of Coastal Agricultural Science,Guangdong Ocean University,Zhanjiang,524088;National Field Genebank for Tropical Fruit,Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture,Institute of South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Science,Zhanjiang,524091;College of Chemistry and Environment,Guangdong Ocean University,Zhanjiang,524088)

机构地区：[1]广东海洋大学滨海农业学院,湛江524088 [2]中国热带农业科学院南亚热带作物研究所,农业部热带果树生物学重点实验室,国家热带果树种质资源圃,湛江524091 [3]广东海洋大学化学与环境学院,湛江524088

出　　处：《分子植物育种》2021年第11期3506-3514,共9页Molecular Plant Breeding

基　　金：广东省自然科学基金项目(2018A030310057,2020A1515011570);广东省教育厅项目(2019KTSCX059);广东海洋大学“南海青年学者”项目(002029001012);广东海洋大学博士启动经费项目(R17023;R19031)共同资助。

摘　　要：为了初步研究大豆(Glycine max)天然抗性相关巨噬蛋白(natural resistance associated macrophage protein,Nramp)家族基因的功能,本研究克隆了一个大豆GmNramp成员基因GmNramp2a。生物信息学分析表明,GmNramp2a基因包含4个外显子,开放阅读框长1551 bp。Gm Nramp2a蛋白含有10个跨膜结构域和13个保守基序,进化树分析表明GmNramp2a蛋白与拟南芥AtNramp3和AtNramp4同源性最高。通过拟南芥原生质体亚细胞定位分析表明,Gm Nramp2a蛋白定位于液泡膜。用qRT-PCR分析GmNramp2a基因的表达模式表明,Gm Nramp2a基因在根、根瘤、叶片和花中都有表达,其中在根瘤和叶片中表达量较高。结果为进一步研究Gm Nramp2a基因功能提供数据参考。In order to preliminarily study the function of soybean(Glycine max)Nramp family gene,a soybean GmNramp member gene,GmNramp2 a,was cloned in this study.Bioinformatics analysis showed that GmNramp2 a gene contained 4 exons,and the length of the open reading frame was 1551 bp.Gm Nramp2 a protein contained 10 transmembrane domains and 13 conservative motifs.Phylogenetic tree analysis showed that GmNramp2 a protein has the highest homology with Arabidopsis AtNramp3 and AtNramp4.Subcellular localization analysis of GmNramp2 a through Arabidopsis protoplast showed that GmNramp2 a protein was localized on vacuolar membrane.Further analysis of the expression pattern of GmNramp2 a gene by fluorescence quantitative RT-PCR showed that GmNramp2 a could express in roots,nodules,leaves and flowers,and the expression level was higher in nodules and leaves.The results of this study will provide data reference further research on the function of GmNramp2 a gene.

关 键 词：大豆(Glycine max) GmNramp2a 基因克隆 亚细胞定位 

分 类 号：S565.1[农业科学—作物学]