植物激素对橡胶树热激转录因子家族成员表达的影响  

Effects of Phytohormones on the Expression of Heat Shock Transcription Factor(Hsf)Family Members in Rubber Tree

作　　者：李言[1] 余文才[2] 陆庆志 王新龙[1] 田维敏[1] Li Yan;Yu Wencai;Lu Qingzhi;Wang Xinlong;Tian Weimin(Hainan Key Laboratory for Cultivation&Physiology of Tropical Crops,Rubber Research Institute,Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P.R.China,Chinese Academy ofTropical Agricultural Sciences,Haikou,571101;Yunnan Institute ofTropical Crops,Jinghong,666100;Chinese Academy ofTropical Agricultural Sciences Proving Ground,Danzhou,571737)

机构地区：[1]中国热带农业科学院橡胶研究所,农业农村部橡胶树生物学与遗传资源利用重点实验室,海南省热带作物栽培生理学重点实验室,海口571101 [2]云南省热带作物科学研究所,景洪666100 [3]中国热带农业科学院试验场,儋州571737

出　　处：《分子植物育种》2021年第11期3559-3570,共12页Molecular Plant Breeding

基　　金：广东省重点领域研发计划项目(2020B020217002);现代农业产业技术体系建设专项资金项目(CARS-33-YZ1)共同资助。

摘　　要：热激转录因子(heat shock transcription factor,Hsf)是一类重要的转录因子,调控ROS清除酶和热激蛋白(heat shock protein,HSP)的活性,从而提高植物的抗逆性。为了研究Hsf调控橡胶树响应非生物胁迫(低温,干旱等),我们分析了橡胶树HbHsfs基因家族的亚类及亚类成员特征;同时,通过Q-PCR技术分析了Hsf家族在中响应茉莉酸甲酯(methyl jasmonate,Me JA)、脱落酸(abscisic acid,ABA)和乙烯(ethylene,ET)的表达模式。橡胶树Hsf家族30个成员中分别响应MeJA、ABA和ET上调表达的有20、9和22个,其中HbHsfA3b、HbHsfA4b、HbHsfA6a和HbHsfA8a均受其诱导表达,但受激素诱导表达的变化规律与响应的时间和强度相关。橡胶树HbHsfs基因受多种植物激素诱导表达,暗示着基因功能的多样性和复杂性。本研究探索了HbHsfs参与MeJA、ABA、ET等激素介导的信号转导途径,为揭示HbHsfs调控橡胶树对低温、干旱等非生物胁迫的耐受性提供理论依据。Heat shock transcription factor(Hsf)is an important transcription factor,which can regulate the activity of ROS scavenging enzymes and heat shock proteins(HSPs),improving the stress resistance of plants.For studying Hsf regulating the response of rubber tree to abiotic stresses(cold,drought,etc.),the study analyzed the subclass and subclass members of Hb Hsf family identified by the whole genome of rubber tree,and analyzed the expression patterns of 30 members of Hsf family in rubber trees clones’93-114’treated with methyl jasmonate(MeJA),abscisic acid(ABA)and ethylene(ET),respectively.The results showed that 20,9 and 22 HbHsf genes were up-regulated in response to Me JA,ABA and ET treatments,respectively.Among them,HbHsfA3 b,HbHsfA4 b,HbHsfA6 a and HbHsfA8 a responded to the induction of these hormones.However,the change of hormone induced expression was related to the response time and intensity.HbHsf genes respond to the various plant hormones in rubber tree,indicating the diversity and complexity of HbHsf gene functions.This study explored the role of HbHsf genes in MeJA,ABA and ET mediated signal transduction pathways,and provided a theoretical basis for the further study of HbHsfs improving the tolerance of rubber trees to abiotic stresses such as low temperature and drought.

关 键 词：巴西橡胶树(Hevea brasiliensis Muell.Arg.) 热激转录因子 脱落酸 茉莉酸甲酯 乙烯 

分 类 号：S794.1[农业科学—林木遗传育种]