基于nrDNA ITS序列的榧树属植物鉴定及亲缘关系分析  

Identification and Genetic Relationship Analysis of Torreya Based on nrDNA ITS Sequence

作　　者：卓维 刘艳[1] 白娟 向蓉 卢圣鄂 任风鸣[1] Zhuo Wei;Liu Yan;Bai Juan;Xiang Rong;Lu Shenge;Ren Fengming(Chongqing Institute ofMedicinal Plant Cultivation,Chongqing,408435;Economic Crops Technology Extension Station of Chongqing Yubei District,Chongqing,401120;Yunyang Saifeng Agricultural Development Co.,Ltd.,Chongqing,404500)

机构地区：[1]重庆市药物种植研究所,重庆408435 [2]重庆市渝北区经济作物技术推广站,重庆401120 [3]云阳县赛凤农业开发有限公司,重庆404500

出　　处：《分子植物育种》2021年第11期3579-3587,共9页Molecular Plant Breeding

基　　金：重庆市基本科研业务费项目(19KF10-2012)资助。

摘　　要：为评价nrDNA ITS序列对榧树属(Torreya)植物的鉴定能力,本研究利用ITS序列和4种不同的分析方法(BLAST比对,K2P遗传距离,SNP位点分析及邻近NJ树)对榧树属植物进行物种鉴定,建立系统进化树讨论其亲缘关系。结果显示,48条ITS序列长度为1095~1105 bp,种内平均遗传距离为0.0016,种间平均遗传距离为0.0150。在物种水平,BLAST比对和NJ树对榧树属植物鉴定效率最高,SNP位点分析可有效鉴定香榧和榧树。亲缘关系分析显示,榧树属其他各种均呈单系分支,但云南榧树与巴山榧树在聚类树中聚为一支,表明二者亲缘关系极近,为近年提出云南榧树并入巴山榧树提供了核基因组序列的佐证。本研究表明ITS序列可作为榧树属物种鉴定的DNA条形码,为榧树属物种鉴定和系统关系提供参考。In order to evaluate the identification ability of nrDNA ITS sequences on Torreya,this study used ITS sequence and four different analysis methods(BLAST,K2 P genetic distance,SNP analysis,NJ tree)to identify species of Torreya,and established phylogenetic trees to discuss their phylogenetic relationships.The results showed that the length of 48 ITS sequences was 1095~1105 bp,and the average intraspecies genetic distance was0.0016 and the average interspecies genetic distance was 0.0150.At the species level,BLAST alignment and NJ tree had the highest efficiency in Torreya.The SNP loci analysis could effectively identify the genus T.grandis cv.’Merrillii’and T.grandis.The analysis of kinship showed that all the other species of the Torreya were single-line branches,but the quince tree of T.yunnanensis and T.fargesii were clustered into a cluster in the ML tree,indicating that the two were closely related.This provided evidence for the nuclear genome sequence proposed the incorporation of T.yunnanensis and T.fargesii.This study showed that the ITS sequence could be used as a DNA barcode for the identification of Torreya,and providing reference for the identification and systematic relationship of Torreya.

关 键 词：DNA条形码 榧树属 ITS序列 鉴定 

分 类 号：S791.53[农业科学—林木遗传育种]