SRAP和CDDP标记在巴戟天遗传多样性分析中的应用  

Application of SRAP and CDDP Markers in Genetic Diversity Analysis of Morinda officinalis How

作　　者：罗铭欣 刘凤民 张伟丽 崔均涛 利冬慧 陈纪言 董章勇 Luo Mingxin;Liu Fengmin;Zhang Weili;Cui Juntao;Li Donghui;Chen Jiyan;Dong Zhangyong(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,510225;Management Center of Teaching and Research Base,Zhongkai University of Agriculture and Engineering,Guangzhou,510225;Deqing Zhongkai Agricultural Tech.Innovation Research Co.,Ltd.,Zhaoqing,526642;College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,510225)

机构地区：[1]仲恺农业工程学院农业与生物学院,广州510225 [2]仲恺农业工程学院教学科研基地管理中心,广州510225 [3]德庆仲恺农业产业科技创新研究有限公司,肇庆526642 [4]仲恺农业工程学院园艺园林学院,广州510225

出　　处：《分子植物育种》2021年第11期3661-3669,共9页Molecular Plant Breeding

基　　金：广东省农业农村厅广东省现代产业园项目;肇庆市德庆县科学技术局资助项目(B72020173)共同资助。

摘　　要：分析肇庆地区主要的巴戟天种质资源,明确各个品种之间的亲缘关系,为巴戟天的培育、保护与鉴别提供科学依据。本研究应用SRAP和CDDP分子标记技术对肇庆9份巴戟天材料进行遗传多样性分析,运用NTSYS-pc 2.1软件计算样品间的遗传相似系数,按照UPGMA方法进行聚类分析,并对两种分子标记结果进行比较与分析。28对SRAP引物组合和16条CDDP引物分别扩增得到140条和83条条带,其中多态性条带数分别为68条和63条,多态性比率(PPB)各为48.6%和75.9%,遗传相似系数变化范围各为0.69~0.96和0.53~0.95,平均遗传相似系数各为0.83和0.69。SRAP在遗传相似系数为0.86时与CDDP在遗传相似系数为0.71时均将广宁特型、广宁特嫁接型、本地大叶型、本地小叶型4个品种聚为一类。SRAP和CDDP两种分子标记均可用于巴戟天的遗传多样性分析,但两种分子标记的聚类结果有一定的差异,所以两种标记联合分析能够更加准确地揭示巴戟天种质之间的亲缘关系。且CDDP的聚类结果与两种标记联合分析的结果更吻合,说明CDDP比SRAP更适用于巴戟天遗传多样性分析。The analysis of the main germ plasm resources of Morinda officinalis How.in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation,protection and identification of Morinda officinalis How..SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How.materials in Zhaoqing.The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software,cluster analysis was carried out according to UPGMA method,and the results of two kinds molecular markers were compared and analyzed.28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively,of which the number of polymorphic bands was 68 and 63 respectively,the polymorphism ratio(PPB)was 48.6%and 75.9%respectively,the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95,and the average genetic similarity coefficient was 0.83 and 0.69 respectively.When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71,the four varieties of Guangning special type,Guangning special grafting type,local large leaf type and Local lobular type were grouped into one group.SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How..There are some differences between SRAP and CDDP,so the combined analysis of the two kinds markers can more accurately reveal the genetic relationship between Morinda officinalis How.germplasm.The results of CDDP clustering were more consistent with those of combined analysis,indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How.than SRAP.

关 键 词：巴戟天 SRAP CDDP 遗传多样性 聚类分析 

分 类 号：S567.239[农业科学—中草药栽培]