冰灯玉露体细胞胚诱导及其形态学和解剖学分析  

Morphological and Histological Analysis of Somatic Embryogenesis in Haworthia cooperivar

作　　者：朱洁琼 赵小梅[1] 林光哲[1] 闫丽[1] 仝德彬 廉玉姬[1] Zhu Jieqiong;Zhao Xiaomei;Lin Guangzhe;Yan Li;Tong Debin;Lian Yuji(College of Life Sciences,Linyi University,Linyi,276000;College of Life Sciences,Shandong Normal University,Jinan,250014;Linyi Xianzhu Biotechnology Co.,Ltd.,Linyi,276000)

机构地区：[1]临沂大学生命科学学院,临沂276000 [2]山东师范大学生命科学学院,济南250014 [3]临沂市仙株生物科技有限公司,临沂276000

出　　处：《分子植物育种》2021年第11期3679-3686,共8页Molecular Plant Breeding

基　　金：临沂大学委托项目(No.HX160402)资助。

摘　　要：以多肉冰灯玉露(Haworthia cooperivar)叶片为外植体,诱导、增殖胚性愈伤组织和植株再生;用Spurr树脂包埋组织切片技术对颗粒状的胚性愈伤组织进行形态学和组织结构特征进行研究。结果表明,外植体在改良的MS+1.5 mg/L 6-BA+0.5 mg/L IAA培养基上可诱导出淡黄色、颗粒状的胚性愈伤组织,其诱导率高达66.7%。胚性愈伤组织在MMS+1.5 mg/L 6-BA+0.4 mg/L IAA培养基上增殖效果最佳,其净增殖量高达14.73 g。胚性愈伤组织上分化的胚状体为乳白色球形或心形结构,由细胞核大、胞质浓密、内含淀粉颗粒的、分裂旺盛特征的细胞构成。内部的胚性愈伤组织呈螺旋状或椭圆形。这些愈伤组织在添加0.1~0.2 mg/L IAA的MMS培养基上进一步发育形成体细胞胚,并在愈伤组织表面分化形成完整的丛生小植物体,体细胞胚在MMS+0.1 mg/L IAA培养基上再生形成根系粗壮的完整的小苗。小苗经驯化后移栽,成活率达100%。该研究为冰灯玉露快速繁殖与分子育种提供了技术支撑。The fleshy leaves were used as materials for induction and proliferation somatic embryonic callus and plant regeneration.The morphological and histological characteristics of granular embryonic callus were studied by light microscopy using Spuur resin section.The results showed that the yellowish and granular embryonic callus was induced on modified MS medium were supplemented with 1.5 mg/L 6-BA and 0.5 mg/L IAA,the highest induction ratio was 66.7%.The highest proliferation net weight(14.73 g)was presented at modified MS medium were added 1.5 mg/L 6-BA and 0.4 mg/L IAA.Milky white globular or heat shape somatic embryo was differentiated from calls that originated from outer or inner-epidermis,the embryonic callus was composed of cells with big nuclear,vigorous division and containing starch granules.The inner embryonic cell clusters showed spiral or oval shapes.These structure further developed to somatic embroy,somatic embryo converted to clumps of small plantlet on surface of callus.Healthy developed seedlings rooted on MMS+0.1 mg/L IAA.This resea-rch provides technical support for the rapid propagation and molecular breeding of Haworthia cooperivar.

关 键 词：冰灯玉露 体细胞胚 解剖学研究 植株再生 

分 类 号：S682.33[农业科学—观赏园艺]