文昌锥种质资源叶片表型多样性分析  

The Phenotypic Diversity Analysis of Castanopsis wenchangensis Germplasm Leaves

作　　者：陈伟文 夏腾飞 孙秀秀 杨立荣 陈加利 戚华沙 王春梅 马光耀 梁振儒 郑道君 Chen Weiwen;XiaTengfei;SunXiuxiu;Yang Lirong;Chen Jiali;Qi Huasha;Wang Chunmei;Ma Guangyao;Liang Zhenru;Zheng Daojun(The Key Laboratory of Tropic Special Economic Plant Innovation and Utilization,National Germplasm Resource Chengmai Observation and Experiment Station,Institute of Tropical Horticulture Research,Hainan Academy of Agricultural Sciences,Haikou,571100;Forest Seed and Seedling General Station of Hainan Province,Forestry Department of Hainan Province,Haikou,570203)

机构地区：[1]海南省农业科学院热带园艺研究所,国家种质资源澄迈观测实验站,海南省特种经济植物种质资源创新利用重点实验室,海口571100 [2]海南省林业局海南省林木种子(苗)总站,海口570203

出　　处：《分子植物育种》2021年第11期3800-3809,共10页Molecular Plant Breeding

基　　金：海南省重点研发计划项目(ZDYF2018076);2020年度海南省省属科研院所技术开发研究专项“热带特色经济植物种质资源收集、保存与评价”;农业农村部物种品种资源保护项目(111821301354052033)共同资助。

摘　　要：文昌锥是海南文昌特有锥属植物,地理分布小且现存数量少。为了解文昌锥叶片表型变异和遗传多样性,本研究对文昌锥7个自然居群叶片的9种叶片表型性状,采用模糊数学的方法进行数量遗传分析。结果表明,文昌锥9种叶片表型性状多样性十分丰富,Shannon-weaver多样性指数变幅为1.96~2.10,均值为2.05;文昌锥叶片表型变异在居群内较高,但是表型变异主要来源于自然居群间;CY、BC、CBX、LFT和MS是选择大叶文昌锥的优势自然居群,XS是选择小叶文昌锥的优势自然居群;文昌锥叶片表型性状间相关性很高,主成分分析把相关性极显著的文昌锥叶片表型指标可以综合概括成叶片大重、叶形指数叶厚、叶柄长以及叶薄等四个主成分因子。本研究可以为文昌锥资源保护、开发利用以及未来分子生物学和基因组学方面的研究提供支持。Castanopsis wenchangensis,with limited distribution area and few existent numbers,is a kind of Castanopsis that is only found in Wenchang city,Hainan province.To explore the nine phenotypic traits variations and genetic diversity of C.wenchangensis leaves,fuzzy mathematic methods were used to do quantitative genetic analysis.The analyzed results demonstrated that variations of leaves phenotypic traits were abundance,with shannon-weaver index range from 1.96 to 2.10 with mean of 2.05.The variations of leaves phenotypic traits in natural populations of C.wenchangensis were also rich,but the main source of variations were detected among those natural populations;CY,BC,CBX,LFT and MS were dominant natural populations for selection of large leaves materials,while XS was suited for small leaves materials selection;the correlation coefficients between the nine leaf phenotypic traits were significantly related,and principal component analysis summarized them into four principal components,including leaf large and weight factor,leaf shape index and thickness factor,petiole length factor and thin leaf factor.Consequently,this study providing useful support information of protection,exploiting and future molecular biology and whole genome studies in C.wenchangensis.

关 键 词：文昌锥(Castanopsis wenchangensis) 叶片表型 多样性 相关分析 主成分分析 

分 类 号：S792.17[农业科学—林木遗传育种]