哈茨木霉124D可湿性粉剂的急性毒理研究  

Evalution on Acute Toxicity of Trichoderma harizanum 124D Wettable Powder

作　　者：李玲[1] 李俊辉 隋丽娜 陈凯 杨凯[1] 王贻莲 刘宝军 李纪顺 LI Ling;LI Jun-hui;SUI Li-na;CHEN Kai;YANG Kai;WANG Yi-lian;LIU Bao-jun;LI Ji-shun(Qilu University of Technology(Shandong Academy of Sciences)Ecology Institute,Shandong Provincial Key Lab for Applied Microbiology,Jinan 250103,China)

机构地区：[1]齐鲁工业大学(山东省科学院)山东省科学院生态研究所山东省应用微生物重点实验室,济南250103

出　　处：《农药》2021年第6期420-423,435,共5页Agrochemicals

基　　金：山东省重点研发计划(2019GSF107066);山东省自然科学基金项目(ZR2020QC044,ZR2019PC060);“十三五”国家重点研发计划(2017YFD0201102,2017YFD0201700);2019年度山东省重点研发计划(重大科技创新工程竞争择优类)(2019JZZY010718);山东省科学院国际科技合作项目(2019GHZD11,2019GHPY05)。

摘　　要：[目的]评价哈茨木霉124D可湿性粉剂在生产和应用中的安全性。[方法]对124D可湿性粉剂进行大鼠急性经口、急性经皮和小鼠急性经呼吸道毒性试验,以及家兔眼刺激和大鼠皮肤刺激性试验,并且应用PCR方法验证哈茨木霉124D的急性经口致病性。[结果]与对照组相比,不同处理方式下的染毒组老鼠和家兔均未出现中毒症状及死亡现象,动物体重与对照相比无显著性差别(P>0.05),并且解剖各脏器均未发现病变部位;经口染毒第1、7、14天后经PCR检测大鼠的重要脏器中均未发现木霉菌目的基因表达。[结论]表明哈茨木霉124D可湿性粉剂对哺乳动物无致病性,该研究为其作为绿色生物农药的安全生产和田间使用提供了依据。[Aims]The study aims to evaluate the safety in production and application of Trichoderma harizanum 124 D wettable power.[Methods]The acute oral in rats,dermal toxicity and in rats,acute inhalation in rats,eye irritation in rabbits and skin irritation in rats were carried out,meanwhile PCR method was applied to detect the acute oral pathogenicity.[Results]Compared with the control,no poisoning symptom or death occurred to the rats and rabbits and there was no significant difference in the body weight of animals in exposure group of different treatments(P>0.05).No pathological changes in gross dissection was found after anatomy between the exposure animals and the control.No target gene of T.harizanum 124 D was found in the vital organs of rats on the 1 st,7 st and 14 st days.[Conclusions]This results suggested that 124 D wettable power had no pathogenicity to mammals,and this research provides basis for production and field practice safety as a green biological pesticide.

关 键 词：哈茨木霉124D 可湿性粉剂 急性毒性 PCR扩增 生物安全 

分 类 号：TQ450.2[化学工程—农药化工]