清浊祛毒丸对淋证模型大鼠膀胱功能及T淋巴细胞亚群的影响  

Effect of Qingzhuo Qudu Pill on Bladder Function and T Lymphocyte Subsets in Rats with Stranguria

作　　者：韦品清 覃孟 曾宪凤 滕红丽 杨旭峰 王静[3] 许艺飞 WEI Pinqing;QIN Meng;ZENG Xianfeng;TENG Hongli;YANG Xufeng;WANG Jing;XU Yifei(Guangxi Qingzhipin Pharmaceutical Co.,Ltd.Yulin 537500 Guangxi,China;Guangxi International Zhuang Medical Hospital,Nanning 530201 Guangxi,China;School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China)

机构地区：[1]广西清之品制药有限责任公司,广西玉林537500 [2]广西国际壮医医院,广西南宁530201 [3]广州中医药大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2021年第6期771-775,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广西科技重大专项(桂科AA18126004)。

摘　　要：目的观察清浊祛毒丸对淋证模型大鼠膀胱功能及T淋巴细胞亚群的影响方法将6周龄SD雌性大鼠60只,随机分为正常组(10只)和造模组(50只);采用膀胱内注射大肠杆菌复制淋证大鼠模型造模后,将造模大鼠随机分为模型组、三金片组(阳性药,2.63g·kg^(-1))以及清浊祛毒丸高、中、低剂量组(5.43、2.71、1.36g·kg^(-1)),每组10只;按对应剂量灌胃给药,每日1次,连续4d,连续给药3d后,对各组大鼠进行尿动力学检测;连续给药4d后,进行外周血T淋巴细胞亚群分析结果与正常组相比,模型组大鼠的膀胱最大容量(MBC)、排尿效率(EV)、顺应性(BC)显著降低(P<0.01),漏尿点压(BLPP)、最大膀胱排尿压(MVP)、排尿前非排尿收缩次数(NVCs)显著增加(P<0.01);CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)明显降低(P<0.05,P<0.01),CD8^(+)显著升高(P<0.05).与模型组相比,清浊祛毒丸各剂量组大鼠的BLPP、MVP、NVCs明显降低(P<O.05,P<0.01),清浊祛毒丸中剂量组大鼠的MBC、EV明显升高(P<0.05),清浊祛毒丸中、低剂量组大鼠的BC明显升高(P<0.05,P<0.01),清浊祛毒丸各剂量组的CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)明显升高(P<0.05,P<0.01),清浊祛毒丸中、低剂量组的CD8^(+)显著降低(P<0.01)。结论清浊祛毒丸能够改善淋证模型大鼠的膀胱功能及T淋巴细胞亚群的异常变化。Objective This study aimed to investigate the effect of Qingzhuo Qudu pill(QZW)on bladder function and T lymphocyte subsets in rats with urinary tract infection.Methods In this study,60(six-week-old)SD female rats were randomly divided into normal group(n=10)and model group(n=50).The rat model of stranguria was established by injecting E.coli into the bladder.After modeling,the model rats were randomly divided into model group,Sanjm tablet group(positive drug,2.63 g•kg^(-1)),and Qingzhuo Qudu pill high,medium,and low dose groups(5.43,2.71,1.36 g•kg^(-1)),10 rats in each group.Rats were gavaged the drugs according to the corresponding doses,once a day for 4 consecutive clays.On the tliird day of continuous administration,the rats in each group were tested for urodynamics.On the fourth day of continuous administration,the T lymphocyte subsets of rats were analyzed.Results Compared with control group rats,the model rats'maximum bladder capacity(MBC),urine efficiency(EV),and bladder compliance(BC)decreased significantly(P<0.01);the bladder leak point pressures(BLPP),the maximum voiding pressure(MVP),and the frequency of non-voiding contractions(NVCs)increased significantly(P<0.01).The CD3^(+),CD4^(+),CD4^(+)/CD8^(+)decreased significantly(P<0.05,P<0.01),CD8^(+)increased significantly(P<0.05).Compared with the model group,the BLPP,MVP and NVCs of the rats in each dose group of the Qingzhuo Qudu pills were significantly reduced(P<0.05,P<0.01),and the MBC and EV of the rats in the Qingzhuo Qudu pill medium dose group were significantly increased(P<0.05),the BC of rats in the medium and low dose groups of Qingzhuo Qudu pill was significantly increased(P<0.05,P<0.01);CD3^(+),CD4^(+),CD4^(+)/CD8^(+)in each dose group of Qingzhuo Qudu pill were significantly increased(P<0.05,P<0.01),and the CD8^(+)levels of the medium and low dose groups of Qingzhuo Qudu pill were significantly decreased(P<0.01).Conclusion QZW can improve bladder function and extraordinarily change T lymphocyte subsets in rats with urinary tract infecti

关 键 词：清浊祛毒丸 淋证 大鼠 尿路感染 尿动力学检测 T淋巴细胞亚群 

分 类 号：R285.5[医药卫生—中药学]