低盐度对澳洲龙纹斑生长、血液及非特异性免疫酶的影响  

Effects of low salinity on the growth,blood and non-specific immune enzymes of Maccullochella peelii peelii

作　　者：赵悦 孙大川[1,2] 刘文畅[1,2] 谭洪新[1,2,3] 罗国芝[1,2,3] 叶继良 ZHAO Yue;SUN Dachuan;LIU Wenchang;TAN Hongxin;LUO Guozhi;YE Jiliang(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [2]上海海洋大学农业农村部淡水水产种质资源重点实验室,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2021年第3期435-441,共7页Journal of Shanghai Ocean University

基　　金：上海水产养殖工程技术研究中心能力提升项目(19DZ2284300);上海市科学技术委员会项目(16DZ2281200)。

摘　　要：研究低盐度对澳洲龙纹斑(Maccullochella peelii peelii)生长、血液生化、非特异性免疫能力的影响。将澳洲龙纹斑盐度驯化后,分别饲养于盐度为0、3、6、9的4组水体中60 d。结果表明,鱼体末体质量随盐度升高显著降低(P<0.05),饲料系数在盐度0组达到最优(1.11±0.11),成活率在盐度3组最高(98.33%±2.89%)。血液生化结果显示:随着盐度上升,肌酐含量呈逐渐降低趋势;尿素氮含量随盐度升高而上升,且在盐度9组[(7.00±0.26)mmol/L]显著高于0组(P<0.05);其他指标均无显著性差异。非特异性免疫酶活力受盐度影响明显,其中碱性磷酸酶和溶菌酶活性均在盐度3组最高,超氧化物歧化酶活性在盐度6组最高,并显著高于其他组;盐度0组和6组的过氧化氢酶活力显著高于其他两组。综上,澳洲龙纹斑适宜在盐度为3的条件下养殖。The effects of low salinity on the growth performance,serum biochemical indices,and non-specific immunity of Maccullochella peelii peelii were evaluated.After acclimating to different salinities,Maccullochella peelii peelii was cultured in four experimental groups for 60 days with salinities of 0,3,6,and 9,respectively.The results showed that:the final body weight was significantly decreased with the increasing salinity(P<0.05);Additionally,the feed conversion ratio attained an optimum value when the salinity was 0(1.11±0.11);and the survival rate was the highest when the salinity was 3(98.33%±2.89%).The serum indices showed that:Creatinine levels followed a decreasing trend with the increasing salinity;Urea nitrogen content increased with the increasing salinity levels,i.e.in the salinity 9[(7.00±0.26)mmol/L],which was significantly higher than that of group 0(P<0.05).Notably,there were no significant differences in other indicators evaluated.The activities of non-specific immune enzymes were significantly affected by the salinity.The activities of alkaline phosphatase and lysozyme were the highest when the salinity was 3.Also,the activity of superoxide dismutase was the highest when the salinity was 6,which was significantly higher than that in other groups.The catalase activities of groups 0 and 6 were significantly higher than that in other groups.In summary,the water salinity level of 3 was found to be suitable for the cultivation of Maccullochella peelii peelii.

关 键 词：澳洲龙纹斑 盐度 生长指标 血清生化 非特异性免疫酶 

分 类 号：S917[农业科学—水产科学]