扶正消积胶囊对胃癌细胞BGC-823的增殖抑制及凋亡的影响  

Effect of Fuzheng Xiaoji Capsule on Proliferation Inhibition and Apoptosis of Gastric Cancer Cell BGC-823

作　　者：薛刚[1] 刘杰[1] 许利军 石建[1] 凌博凡 XUE Gang;LIU Jie;XU Lijun;SHI Jian;LING Bofan(Rugao Hospital of Traditional Chinese Medicine,Nanjing University of Chinese Medicine,Rugao 226500,China;Jiangsu Chinese Medicine Hospital,Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属如皋市中医院,如皋226500 [2]南京中医院大学附属江苏省中医院,南京210029

出　　处：《世界中医药》2021年第10期1526-1529,1533,共5页World Chinese Medicine

基　　金：国家中医药管理局基地开放课题(JD2019SZ05);南通市青年医学人才项目(青年078)。

摘　　要：目的:研究扶正消积胶囊在体外对人胃癌细胞BGC-823增殖抑制及凋亡的影响。方法:MTT实验检测扶正消积胶囊对BGC-823细胞增殖的影响;流式细胞仪检测其对BGC-823细胞凋亡的影响;Real Time-PCR、Western blotting实验检测其凋亡相关基因表达。结果:与空白组比较,BGC-823细胞的增殖能被扶正消积胶囊显著抑制(给药24 h),此抑制率随给药剂量的增加而上升,在剂量为5 mg/mL时,其抑制率达86.71%,且这种抑制呈剂量依赖关系。Annexin V/PI双染法联合流式细胞仪结果显示扶正消积胶囊能诱导BGC-823细胞凋亡,BGC-823细胞早期凋亡率随给药剂量的增加,在给药剂量分别为2.5 mg/mL、5 mg/mL时,BGC-823细胞早期凋亡率分别达到15.76%、38.25%;Western blotting、Real Time-PCR结果表明,Procaspase-3、9蛋白酶表达随剂量的增加而明显减小,Bcl-2、Bcl-xl、Survivin 3种基因表达随给药剂量的增加而下降,Bax基因表达则相反。结论:扶正消积胶囊能通过激活caspase级联反应抑制胃癌细胞增殖并诱导其凋亡。Objective:To investigate the influence of Fuzheng Xiaoji Capsule on the proliferation inhibition of human gastric cancer cell BGC-823 and its apoptosis.Methods:Effects of Fuzheng Xiaoji Capsule on proliferation of BGC-823 cells were investigated with MTT assay.Flow cytometry was used to detect the cell apoptosis while the effect of apoptosis related gene of Fuzheng Xiaoji capsule on BGC-823 cell was measured by real time-PCR and Western blotting.Results:MTT assay showed that the BGC-823 cells were inhibited by Fuzheng Xiaoji Capsule compared with control group,after treated on 24 h.The inhibition rate increases with the increase of dose,which is dose dependent.At the dose of 5 mg/mL,the inhibition rate reached 86.71%(P<0.05).Flow cytometry demonstrated that Fuzheng Xiaoji Capsule could induce the apoptosis of BGC-823 cells.The early apoptosis rate of BGC-823 cells increased with the dose of the drug.At the dose of 2.5 mg/mL and 5 mg/mL,respectively,the early apoptosis of BGC-823 cells reached 15.76%and 38.25%respectively.By the results of Western blotting and real time-PCR,the expression of caspase-3、caspase-9 was significantly lower as the dose increased.Also,it could reduce the activity of Bcl-2,Bcl-xl,Survivin and increase the activity of Bax.Conclusion:Fuzheng Xiaoji Capsule could inhibit the proliferation of BGC-823 cells and induce their apoptosis.The possible mechanism was through activation of caspase cascade reaction.

关 键 词：扶正消积胶囊 BGC-823胃癌细胞 肿瘤 增殖 凋亡 

分 类 号：R273[医药卫生—中西医结合]