子宫腺肌病中差异基因筛选及Wilms’tumor-1的表达  

Identification of differentially expressed genes and Wilms’tumor-1 expression in adenomyosis

作　　者：耿晨 杨阳[1] 赵月 刘浩冉 晁岚[1] GENG Chen;YANG Yang;ZHAO Yue;LIU Haoran;CHAO Lan(Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Qilu Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]山东大学齐鲁医院妇产科不孕不育诊疗中心,山东济南250012

出　　处：《山东大学学报（医学版）》2021年第4期79-86,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81571511);山东省重点研发计划项目(2019GSF107004)。

摘　　要：目的从GEO数据库中筛选子宫腺肌病的差异基因,探讨威尔姆氏瘤基因-1(WT1)基因在子宫腺肌病中的表达。方法通过GEO数据库进行候选基因的筛选;对差异基因进行KEGG和GO富集分析;Western blotting技术检测腺肌病组和对照组中WT1蛋白质表达;实时定量聚合酶链反应(qRT-PCR)检测子宫腺肌病异位内膜组织(腺肌病组)和正常子宫内膜(对照组)的WT1 mRNA表达;免疫组织化学测定WT1基因在腺肌病组及对照组中的表达位置及表达水平。结果差异基因的KEGG和GO富集于转录因子活性,钙黏蛋白结合参与细胞间粘附、生长因子结合等过程。Western blotting技术显示,子宫腺肌病异位内膜组织中WT1蛋白质表达水平低于对照组正常子宫内膜组织(P=0.002)。qRT-PCR结果显示,子宫腺肌病异位内膜组织中WT1 mRNA表达水平低于对照组正常子宫内膜组织(P=0.003)。免疫组织化学染色显示,腺肌病组异位内膜组织中间质WT1染色水平低于对照组(P=0.001);腺肌病组异位内膜组织中腺体WT1染色水平高于对照组(P<0.001);腺肌病组异位内膜组织WT1总体染色水平低于对照组(P=0.003)。结论从GEO数据库筛选得到差异基因并进行KEGG和GO富集分析,WT1在子宫腺肌病异位子宫内膜差异表达可能在子宫腺肌病的发生发展中发挥作用。Objective To screen the differentially expressed genes(DEGs)in adenomyosis from the Gene Expression Omnibus(GEO)database and to explore the expression of Wilm?s tumor-1(WT1).Methods The candidate genes were screened in the GEO database.The DEGs underwent Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment.The protein and mRNA expressions of WT1 in adenomyosis group and control group were detected with Western blotting and real-time quantitative polymerase chain reaction(qRT-PCR),respectively.The expression position and expression of WT1 in adenomyosis group and control group were determined with immunohistochemical staining.Results KEGG and GO of DEGs were enriched in transcription factor activity,cadherin binding involved in cell-cell adhesion,growth factor binding and other processes.Western blotting and qRT-PCR showed that the protein and mRNA expressions of WT1 were lower in the adenomyosis group than in the control group(P=0.002,P=0.003).Immunohistochemical staining showed that the WT1 staining level in stroma was lower in the adenomyosis group than in the control group(P=0.001),while the WT1 staining level in epithelium was higher(P<0.001);the overall staining level of WT1 in the adenomyosis group was lower than that in the control group(P=0.003).Conclusion The DEGs are screened from the GEO database and underwent KEGG and GO enrichment and WT1 may be involved in the pathogenesis of adenomyosis.

关 键 词：子宫腺肌病 GEO数据库分析 差异基因 富集分析 Wilms’tumor-1基因 

分 类 号：R711.71[医药卫生—妇产科学]