DNA/RNA甲基化修饰检测技术进展  

Advances in the detection technology of DNA/RNA methylation

作　　者：张耿 边佳辉 崔亚宁 李晓娟 ZHANG Geng;BIAN Jia-hui;CUI Ya-ning;LI Xiao-juan(Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing 100083;National Engineering Laboratory for Tree Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林木分子设计育种高精尖创新中心,北京100083 [2]北京林业大学生物科学与技术学院林木育种国家工程实验室,北京100083

出　　处：《电子显微学报》2021年第3期348-359,共12页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31871349);北京林业大学“杰出青年人才”培育计划(No.2019JQ03003)。

摘　　要：表观遗传学研究的是在不改变基因序列前提下,持续性和可遗传性改变基因活性状态的分子和机制。其中DNA和RNA的修饰是近几年表观遗传修饰研究的热点。为了对这些表观遗传修饰的动态分布、机制和功能有更深入的认识和理解,研究人员开发了多种定量和定位分析的检测方法。本文概述了DNA和RNA甲基化修饰形成的机制以及在植物中的功能,重点综述了近年来建立的检测表观遗传修饰的技术,并展望了新兴的单细胞和单分子测序技术的发展。高通量高精度检测技术的突破性发展,将极大促进对植物中表观遗传修饰的研究。Epigenetics research involves the study of molecules and mechanisms,which can heritable change gene activity in the context of the same DNA sequence.The modifications of DNA and RNA have been the focus of epigenetic research over the last few decades.Some techniques and methods have been developed to study the dynamic distribution,molecular mechanisms,and functions of these epigenetic modifications.In this review,we give a brief introduction on the mechanism of DNA and RNA methylation as well as its functions in plants,and mainly focus on the techniques for detecting epigenetic modification.We also discuss the potential of single cell and single-molecule sequencing.The breakthrough advancements in technologies will significantly accelerate the research on epigenetic modification in plants.

关 键 词：表观遗传 DNA修饰 RNA修饰 单细胞测序 单分子测序 

分 类 号：Q943[生物学—植物学] Q942