3株临床分离产OXA-232碳青霉烯耐药肺炎克雷伯菌的流行病学分析  

Epidemiological investigation of three clinically isolated carbapenem-resistant strains of OXA-232-producing Klebsiella pneumoniae

作　　者：付宏煜 谢小芳[1] 范银银[1] 朱志宸 徐塑凯 杜鸿[1] FU Hongyu;XIE Xiaofang;FAN Yinyin;ZHU Zhichen;XU Sukai;DU Hong(Department of Clinical Laboratory, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China)

机构地区：[1]苏州大学附属第二医院检验科,江苏苏州215004

出　　处：《临床检验杂志》2021年第5期390-394,共5页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省卫生健康委医学科研重点项目(ZDB2020014);苏州大学附属第二医院学科托举创新团队项目(XKTJ-TD202001);苏州大学“大学生创新创业训练计划”(201910285134Y)。

摘　　要：目的描述临床OXA-232肺炎克雷伯菌(OXA-232-producing Klebsiella pneumoniae,OXA-232Kp)的流行病学特征。方法收集2018年9月—2019年9月江苏5家医院临床分离的碳青霉烯耐药肺炎克雷伯菌(carbapenem-resistant Klebsiella pneumoniae,CRKP),采用微量肉汤稀释法和Phoenix-M50自动微生物系统进行药敏分析;PCR结合测序检测bla_(OXA-232)基因;脉冲场凝胶电泳(PFGE)分析菌株同源性;质粒测序分析携带bla_(OXA-232)基因的质粒周围环境。结果筛选出3株OXA-232Kp ST15型,PFGE分析呈克隆性传播,携带6141 bp ColKP3型质粒,与中国东部研究发现携带bla_(OXA-232)基因的质粒高度同源。结论本文首次调查OXA-232Kp ST15在江苏地区的流行病学特征。携带bla_(OXA-232)的质粒具有高度的同源性,表明该6141 bp ColE型质粒对OXA-232在江苏地区的流行具有重要作用。Objective To investigate the clinical epidemiological characteristics of OXA-232-producing Klebsiella pneumoniae(OXA-232Kp).Methods The clinical isolates of carbapenem-resistant Klebsiella pneumoniae(CRKP)from 5 hospitals in Jiangsu province during September 2018 and September 2019 were collected,and their drug susceptibility were analyzed by the microbroth dilution method and Phoenix-M50 automatic microbiological system.The bla_(OXA-232) gene was detected by PCR and sequencing.The homology of the strains was analyzed by the pulsed field gel electrophoresis(PFGE).The surrounding environment of the plasmid carrying bla_(OXA-232) gene was analyzed by the plasmid sequencing.Results Three strains of OXA-232Kp ST15 were screened.PFGE analysis showed that they were clonal transmission,and carried the 6141 bp ColKP3 plasmid,which was highly homologous with the plasmid carrying the bla_(OXA-232) gene found in eastern China.Conclusion This is the first time to investigate the epidemiological characteristics of OXA-232Kp ST15 in Jiangsu province.The high homology of the plasmid carrying bla_(OXA-232) indicates that the 6141 bp ColE type plasmid plays an important role in the prevalence of OXA-232 in Jiangsu region.

关 键 词：克隆性传播 肺炎克雷伯菌 OXA-232 质粒 

分 类 号：R446.5[医药卫生—诊断学]