薰衣草花色苷与牛血清蛋白非共价相互作用研究  

Interaction of anthocyanins from Lavender with bovine serum albumin

作　　者：李楠[1] 王国振[1] 曾永明[1] 冯娜 LI Nan;WANG Guo-zhen;ZENG Yong-ming;FENG Na(Department of Chemistry and Applied Chemistry,Changji University,Changji 831100,China)

机构地区：[1]昌吉学院化学与应用化学系,新疆昌吉831100

出　　处：《化学研究与应用》2021年第6期1004-1011,共8页Chemical Research and Application

基　　金：新疆自治区高校科研计划项目(XJEDU2018Y047,XJEDU2019Y056)资助。

摘　　要：采用荧光光谱和紫外-可见光谱法研究了薰衣草花色苷与牛血清白蛋白(BSA)之间的相互作用。结果表明:薰衣草花色苷对BSA有较强的内源荧光猝灭作用,其猝灭机制为静态猝灭,并伴有非辐射能量转移。在298,303和310 K时,荧光猝灭速率常数分别为1.65×10^(4)、0.98×10^(4)和0.78×10^(4)L·mol^(-1)、结合常数(Ka)分别为40680.85、14789.38和7598.01 L·mol^(-1)及对应的结合位点数(n)分别为1.09116、1.06521和0.97505。通过计算热力学参数可推断薰衣草花色苷与BSA之间的主要作用力为氢键和范德华力。同步荧光光谱和紫外-可见光谱表明薰衣草花色苷主要影响BSA氨基酸残基所处的微环境,特别是疏水作用减弱,从而改变了BSA的分子构象。另外,通过加入外源性金属Zn^(2+)离子,进一步证明了以上研究结果。The interaction anthocyanins from Lavender with bovine serum albumin(BSA)was investigated using fluorescence spectroscopy,synchronous fluorescence spectroscopy and absorption spectroscopy under imitated physiological conditions.The data from the fluorescence spectra confirmed that the anthocyanins from Lavender could strongly quench the intrinsic fluorescence of BSA effectively via static quenching,accompanied by non-radiative energy transfer.Various fluorescence quenching rate constants(1.65×10^(4),0.98×10^(4) and 0.78×10^(4)L·mol^(-1)),binding constants(40680.85,14789.38 and 7598.01 L·mol^(-1)),and the corresponding binding sites(1.09116,1.06521 and 0.97505)for the BSA-anthocyanins from Lavender systems were hereby obtained under different temperatures(298,303 and 310 K).The corresponding thermodynamic parameters obtained at different temperatures indicated that hydrogen bond and van der Waals force played a major role in the interaction of anthocyanins and BSA.Synchronous fluorescence spectra and Uv-vis spectra futher demonstrated that the addition of anthocyanins from Lavender impacted the microenvironment around BSA in aqueous solution,especially the decreased hydrophobic effect causing a conformational change of BSA.In addition,the results were further verified by the addition of exogenous metal Zn^(2+)ion.

关 键 词：薰衣草花色苷 牛血清白蛋白 相互作用 金属离子 

分 类 号：O657.3[理学—分析化学]