核-壳型Fe_(3)O_(4)@Au定向固定葡萄糖氧化酶研究  

Study on oriented immobilization of glucose oxidase by core-shell Fe_(3)O_(4)@Au

作　　者：高丰琴[1,3] 刘亭 王晓芳[1] 牟萱 GAO Feng-qin;LIU Ting;Wang Xiao-fang;MOU Xuan(College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China;Experimental High School of NingJiang District,Songyuan 138001,China;School of Chemistry&Chemical Engineering,Shaanxi Normal University,Xi’an 710119,China)

机构地区：[1]咸阳师范学院化学与化工学院,陕西咸阳712000 [2]宁江区实验高级中学,吉林松原138001 [3]陕西师范大学化学化工学院,陕西西安710062

出　　处：《化学研究与应用》2021年第6期1058-1063,共6页Chemical Research and Application

基　　金：陕西省教育厅科研计划项目(16JK1827)资助;咸阳师范学院(国家级)大学生创新创业训练计划项目(S202010722011)资助。

摘　　要：以核-壳型结构的Fe_(3)O_(4)@Au微粒为载体,采用亲和素-生物素系统对葡萄糖氧化酶的固定化进行研究。表征结果表明,固定化酶呈现不规则圆球形,直径为80~180 nm左右。通过单一因素实验,探讨出葡萄糖氧化酶固定化的最优条件:磷酸盐缓冲溶液pH为4、固定化时间为40 min、固定化温度为30℃。游离酶与固定化酶的存贮稳定性表明,固定化葡萄糖氧化酶显示了更好的稳定性,可能与固定酶结构中存在的笼状结构有关。The immobilization of glucose oxidase was fabricated with the carrier of core-shell Fe_(3)O_(4)@Au particles using the specific avidin-biotin interaction.The results showed that the immobilized enzyme showed irregular spherical shape and the diameter of the immobilized enzyme was about 80~180 nm.Through a single factor experiment,the optimal conditions for the immobilization of glucose oxidase were explored:pH 4 in phosphate buffer solution,immobilization time of 40 min and temperature of 30 oC.The storage stability showed that immobilized glucose oxidase showed better stability than free enzyme,which might be related to the cage-like structure of immobilized enzyme.

关 键 词：Fe_(3)O_(4)@Au 葡萄糖氧化酶 固定化 

分 类 号：O629.8[理学—有机化学]