黄芪注射液在大鼠肠缺血再灌注肝损伤中的应用实验  

Application of Astragalus Injection in Intestinal Ischemia-Reperfusion Injury in Rats

作　　者：郝占伟[1] 吴星星 HAO Zhan-wei;WU Xing-xing(The Second People Hospital of Lianyungang,Department of Pediatric Surgery,Lianyungang,222000,China)

机构地区：[1]连云港市第二人民医院儿外科,连云港222000

出　　处：《神经药理学报》2020年第5期1-4,共4页Acta Neuropharmacologica

摘　　要：目的:探究黄芪注射液在大鼠肠缺血再灌注肝损伤(intestinal ischemia-reperfusion,IIR)中的应用情况及对Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)表达水平的影响。方法:将9只成年健康雄性Wistar大鼠随机分为3组,分别为假手术组(Sham组)、模型组(IIR组)和黄芪注射液组(黄芪组),每组3只大鼠,3组按照再灌注时间(1、3、6 h)划分为3个不同时相(每一个时相均为1只大鼠),实验结束后取大鼠肝左叶作为实验标本,利用MIAS-2000医学图像分析系统及免疫组化法检测全部标本Bax、Bcl-2表达水平,利用缺口末端标注技术(TUNEL)检测肝组织中肝细胞凋亡指数(apoptosis index,AI)。结果:与Sham组相比,IIR组与黄芪组3个不同时相Bax、Bcl-2表达水平均明显提高(P<0.05),IIR组3个不同时相Bcl-2/Bax降低,黄芪组3个不同时相Bcl-2/Bax水平均降低(P<0.05)。与IIR组对比,黄芪组再灌注1、3、6 h 3个不同时相Bcl-2/Bax、Bcl-2都显著提高,Bax水平降低(P<0.05)。同组中不同时相间对比无明显差异(P>0.05)。与Sham组相比,IIR组与黄芪组再灌注1、3、6 h 3个不同时相AI均显著提高(P<0.05)。与IIR组对比,黄芪组3个不同时相AI都显著降低(P<0.05)。IIR组与黄芪组同组中不同时相间对比具有明显差异(P<0.05),而且再灌注6 h最高。结论:大鼠发生IIR后可导致肝损伤,而且Bcl-2、Bax表达水平提高,黄芪注射液的作用机制可能在于调节两种表达,缓解肝细胞凋亡,具有肝保护效果。Objective:To investigate the application of Astragalus injection in intestinal ischemia-reperfusion liver injury in rats and its influence on the expression levels of Bax and Bcl-2.Methods:9 healthy adult male Wistar rats were randomly divided into 3 groups:Sham group,model group(IIR group)and Astragalus injection group(Astragalus group),with 3 rats in each group.The 3 groups were divided into 3 different phases(each time phase was 1 rats)according to the time of reperfusion(1,3,6 h).After the end of the experiment,the expression levels of Bax and Bcl-2 in the left lobe of rat liver were taken as the experimental standard,and the expression levels of Bax and Bcl-2 in all specimens were detected by MIAS-2000 medical image analysis and immunohistochemical method,and the apoptosis index(AI)of liver tissues was detected by the TUNEL technique.Results:Compared with Sham group,the expression levels of Bax and Bcl-2 in three different phases of IIR group and Huangqi group were significantly increased(P<0.05),while those of Bcl-2/Bax and Bcl-2/Bax in three different phases of IIR group and Astragalus group were decreased(P<0.05).Compared with the IIR group,the reperfusion of bcl-2/Bax and Bcl-2 in the astragalus group at 1,3 and 6 h was significantly increased,while the level of Bax was decreased(P<0.05).There was no significant difference between different phases in the same group(P>0.05).Compared with Sham group,AI of IIR group and Huangqi group were significantly improved at different phases of reperfusion at 1,3 and 6 h(P<0.05).Compared with IIR group,AI of astragalus group in three different phases was significantly reduced(P<0.05).There were significant differences between IIR group and Astragalus group in different phases(P<0.05),and reperfusion was the highest for 6 h.Conclusion:IIR can lead to liver injury in rats,and the expression levels of Bcl-2 and Bax are increased.The mechanism of action of Astragalus injection may be to regulate the two expressions,alleviate the apoptosis of liver cells,and have a protect

关 键 词：黄芪注射液 大鼠肠缺血再灌注 肝损伤 

分 类 号：R965[医药卫生—药理学]