卷柏总黄酮的质量标准研究  

Research on the quality standard of Selaginella total flavones

作　　者：李彦超[1] 周宁[2] 郑晓珂[2] LI Yanchao;ZHOU Ning;ZHENG Xiaoke(He′nan Institute for Food and Drug Control,National Medical Products Administration Key Laboratory for Quality Control of Traditional Chinese Medicine(Chinese Materia Medica and Prepared Slices),Zhengzhou 450008,China;He′nan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南省食品药品检验所国家药品监督管理局中药材及饮片质量控制重点实验室,郑州450008 [2]河南中医药大学,郑州450046

出　　处：《西北药学杂志》2021年第3期353-356,共4页Northwest Pharmaceutical Journal

基　　金：国家重大新药创制-科技重大专项(编号:2013ZX09102-022)。

摘　　要：目的建立适当的定性、定量检测方法,以期更加有效、准确地控制卷柏总黄酮的质量。方法采用HPLC法对卷柏总黄酮进行定性鉴别;以穗花杉双黄酮为对照,用UV法测定卷柏总黄酮的含量,HPLC法测定穗花杉双黄酮的含量;参照《中国药典》2015年版方法测定重金属及有害元素、有机氯农残和其他检查项。结果卷柏总黄酮和穗花杉双黄酮质量浓度分别在1.56~11.68、6.40~320.03μg·mL^(-1)范围内线性关系良好(r=1.0000),平均回收率分别为98.18%(RSD=1.76%)、96.66%(RSD=1.15%)。重金属及有害元素、有机氯农残和其他检查项均符合规定。结论所建立的方法简便、准确,指标成分科学有效,为该品种的质量评价与控制提供了有力的技术支持。Objective To establish reasonable qualitative and quantitative detection methods of Selaginella total flavones,which can be used to evaluate the quality effectively and accurately.Methods Selaginella total flavones were identified by HPLC.Amentoflavone as the standard,HPLC method was employed to determine the content of amentoflavone and UV method was used to determine the content of Selaginella total flavones.According to the methods recorded in Chinese Pharmacopoeia(2015 edition),heavy metals and harmful elements,organochlorine pesticide residues,and other requirementitems were studied.Results The Selaginella total flavones showed a good linear relationship in the range of 1.56-11.68μg·mL^(-1)(r1=1.0000).The average recovery was 98.18%with RSD 1.76%.Amentoflavone showed a good linear relationship in the range of 6.40-320.03μg·mL^(-1)(r2=1.0000).The average recovery was 96.66%with RSD 1.15%.Heavy metals and harmful elements,organochlorine pesticide residues,and other requirements were all complied with the specification.Conclusion The methods established in the study were simple and accurate,and the chemical composition makers were effective.This paper provides a strong technical support for the quality evaluation and control of Selaginella total flavones.

关 键 词：卷柏总黄酮 穗花杉双黄酮 质量标准 HPLC法 UV法 

分 类 号：R282[医药卫生—中药学]