飞扬肠胃炎胶囊对实验性胃溃疡的保护作用及机制研究  

Study on the protective effect and mechanism of Feiyang Changweiyan Capsules on experimental gastric ulcer

作　　者：周丹 田天 李骅 舒庆 赵小燕 余承莉 耿鑫 王四旺 ZHOU Dan;TIAN Tian;LI Hua;SHU Qing;ZHAO Xiaoyan;YU Chengli;GENG Xin;WANG Siwang(Department of Pharmacy,the Ninth Hospital of Xi′an,Xi′an 710054,China;Department of Chinese Materia Medica and Natural Medicines,School of Pharmacy,Air Force Medical University,Xi′an 710032,China)

机构地区：[1]西安市第九医院药剂科,西安710054 [2]空军军医大学药学系中药与天然药物学教研室,西安710032

出　　处：《西北药学杂志》2021年第3期408-414,共7页Northwest Pharmaceutical Journal

基　　金：陕西省自然科学基础研究计划项目(编号:2021JQ-923);西安市卫生健康委员会中医药科研项目(编号:SZJ202004);西安市科技计划项目(编号:2017121SF/YX015)。

摘　　要：目的探讨飞扬肠胃炎胶囊对实验性胃溃疡(GU)的保护作用及其可能的机制。方法将48只SD大鼠随机分为正常组、模型组、兰索拉唑组以及飞扬肠胃炎胶囊低、中及高剂量组(200、400、600 mg·kg^(-1)),每组8只。采用无水乙醇灌胃,建立大鼠GU模型。对大鼠的溃疡指数(UI)进行评分,测定胃内容物酸度(pH)、胃黏液量(GWM),HE染色观察胃黏膜的组织病理学改变,Elisa法检测前列腺素E2(PGE-2)的水平,比色法检测氧化指标过氧化氢酶(CAT)、超氧化物岐化酶(SOD)、硫代巴比妥酸反应物(TBARS)、谷胱甘肽(GSH)和一氧化氮(NO)的水平,免疫组化法检测核因子NF-E2相关因子(Nrf2)和血红素加氧酶1(HO-1)的蛋白表达。结果与模型组比较,飞扬肠胃炎胶囊各剂量组与兰索拉唑组UI显著降低,胃内容物pH值升高,GWM增加,PGE-2、CAT、SOD、GSH和NO的水平均增强,TBARS水平降低,且飞扬肠胃炎胶囊低剂量组与兰索拉唑组之间差异有统计学意义(P<0.05)。此外,飞扬肠胃炎胶囊中、高剂量组均可明显改善胃组织病理学改变,提高Nrf2和HO-1的蛋白表达。结论飞扬肠胃炎胶囊对实验性GU具有显著保护作用,且其可能的作用机制为调控Nrf2/HO-1信号通路的表达。Objective To study the protective effect and possible mechanism of Feiyang Changweiyan Capsules on experimental gastric ulcer(GU).Methods 48 SD rats were randomly divided into normal group,model group,lansoprazole group,Feiyang Changweiyan Capsules low,middle and high dose groups(200,400 and 600 mg·kg^(-1)),with 8 rats in each group.The rat GU model was established by intragastric administration of absolute alcohol.The ulcer index(UI)of rats was scored,the gastric contents were collected to determine pH,and the gastric mucus volume(GWM)was measured.The histopathological changes of gastric mucosa were observed by HE staining,the levels of prostaglandin E2(PGE-2)was detected by Elisa,and the activities of oxidation indexes including catalase(CAT),superoxidase dismutase(SOD),thiobarbituric acid reactive substances(TBARS),glutathione(GSH)and nitric oxide(NO)were monitored by colorimetry.The expression of nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase 1(HO-1)protein was detected by immunohistochemistry.Results Compared with model group,each Feiyang Changweiyan Capsules groups and lansoprazole group could significantly decrease UI,increase pH in stomach,GMW,PGE-2,CAT,SOD,GSH and NO levels,and decrease TBARS levels.There was significant difference between Feiyang Changweiyan Capsules low dose group and lansoprazole group(P<0.05).In addition,middle and high dose of Feiyang Changweiyan Capsules groups could significantly improve the gastric histopathological changes,and increase the expression of Nrf2 and HO-1 protein.Conclusion Feiyang Changweiyan Capsules has obviously protective effect on experimental GU and the possible mechanism is regulating the expression of Nrf2/HO-1 signal pathway.

关 键 词：飞扬肠胃炎胶囊 胃溃疡(GU) 氧化应激 核因子NF-E2相关因子(Nrf2)/血红素加氧酶1(HO-1)信号通路 

分 类 号：R965[医药卫生—药理学]