不同参数ALA-PDT对皮肤鳞癌A431细胞的体外杀伤抑制作用  

Inhibition of Skin Squamous Carcinoma A431 Cells with PDT of Different Parameters

作　　者：鲍丽 蔡宏 刘玮[1,2] BAO Li;CAI Hong;LIU Wei(China Medical University,Shenyang 110001,China;Air Force Medical Center of PLA)

机构地区：[1]中国医科大学,沈阳市110001 [2]空军特色医学中心

出　　处：《中国激光医学杂志》2021年第2期61-64,117,共5页Chinese Journal of Laser Medicine & Surgery

基　　金：北京市自然科学基金(7202200);国家重点研发计划(2018YFB0407202)。

摘　　要：目的探讨不同参数5-氨基酮戊酸光动力疗法(5-aminolaevulinic acid-based photodynamic therapy,ALA-PDT)对皮肤鳞状细胞癌A431细胞生长的抑制效应。方法实验对象为人皮肤鳞癌细胞系(A431),实验分为对照组和PDT组两个大组,其中对照组再分4个小组:(1)空白对照组,不接种细胞、不加光敏药物和无激光照射;(2)细胞对照组,仅接种细胞,不加光敏药物和不行激光照射;(3)光照对照组,接种细胞后仅激光照射(能量密度:3、6、9、12 J/c㎡)不加光敏药物;(4)药物对照组,接种细胞后加入不同光敏剂浓度(15.625、31.25、62.5、125、250μg/ml)。PDT组再按照不同光敏剂浓度(15.625、31.25、62.5、125、250μg/ml)+不同光照剂量(能量密度3、6、9、12 J/c㎡)分组。各组细胞处理后3 h,采用CCK-8法检测细胞活力计算细胞抑制率,同时采集细胞显微镜下图片。结果单独激光照射及单独使用光敏剂对细胞生长无抑制效应,ALA-PDT对A431细胞的抑制效应受光敏剂浓度以及光照能量密度共同影响。在一定范围内,PDT对细胞的抑制效应随光敏剂浓度、光照剂量的增加而增强,但PDT对细胞的抑制效应不随剂量增加而无限增加。结论合适参数的ALA-PDT处理对A431细胞具有抑制作用,光敏剂浓度62.5μg/ml、能量密度9 J/c㎡时,ALA-PDT对A431细胞的抑制效应达到最佳,继续增加光敏剂浓度或光照能量密度均不能提高PDT的抑制效应。Objective To investigate the inhibitory effect of photodynamic therapy of different parameters on skin squamous carcinoma A431 cells.Methods Human skin squamous cells(A431)were used as the experimental subjects and divided into two groups:the control group and PDT group.The control group was further split into four groups:(1)the blank control group(without cell inoculation,drug or laser irradiation),(2)the cell control group(sole cell inoculation),(3)the light control group[sole laser irradiation(photosensitizer concentration:0,energy density:3,6,9,12 J/c㎡)],and(4)the drug control group[sole drug application(photosensitizer concentration:15.625,31.25,62.5,125,250μg/mL,energy density:0)].The PDT group was further split up by photosensitizer concentrations(15.625,31.25,62.5,125,250μg/mL)and light doses(3,6,9,12 J/c㎡).For each group,CCK-8 assay was used to detect the cell viability 3 h after the treatment,and cell microscope pictures were collected.Results The inhibiting effect of photodynamic therapy on A431 cells was influenced by concentration of photosensitizer and energy density of light.To some extents,the inhibiting effect of photodynamic therapy on cells increased with the increase of photosensitizer concentration and light dose,but the inhibiting effect of photodynamic therapy on cells did not increase indefinitely with the increase of the dose.Conclusions Photodynamic treatment of appropriate parameters has a inhibiting effect on A431 cells.PDT exerts the best inhibiting effect on A431 cells when the photosensitizer concentration is 62.5μg/ml and the energy density is 9 J/c㎡,but further increasing the concentration of photosensitizer or light energy density will not enhance the effect.

关 键 词：光动力疗法 A431细胞 细胞活性 细胞形态 

分 类 号：R616.4[医药卫生—外科学] R739.5[医药卫生—临床医学]