甘露寡糖对阿司匹林诱导大鼠肠道损伤修复作用的研究  

Effects of Mannose Oligosaccharide on Repair of Intestinal Injury Induced by Aspirin in Rats

作　　者：陈勇江 王金荣[1] 乔汉桢 苏兰利[1] 唐桂芬[2] 黄进[1] 赵银丽[1] 高温婷 CHEN Yongjiang;WANG Jinrong;QIAO Hanzhen;SU Lanli;TANG Guifen;HUANG Jin;ZHAO Yinli;GAO Wenting(College of Bioengineering,Henan University of Technology,Zhengzhou 450001,China;Henan University of Animal Husbandry and Economy,Zhengzhou 450011,China)

机构地区：[1]河南工业大学生物工程学院,郑州450001 [2]河南牧业经济学院,郑州450011

出　　处：《动物营养学报》2021年第6期3523-3531,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(U1604106,31702235)。

摘　　要：本试验旨在研究甘露寡糖(MOS)对阿司匹林(ASA)诱导大鼠的肠道损伤修复作用。试验采用单因素试验设计,选用36只6周龄150~180 g大鼠,经过1周适应性饲养后随机分为6组,每组6只,单笼饲养。空白组灌胃生理盐水,模型组灌胃2周200 mg/kg ASA后灌胃1周生理盐水,MOS组灌胃1周生理盐水后灌胃2周600 mg/kg MOS,ASA1、ASA2和ASA3组分别灌胃2周50、100和200 mg/kg ASA后灌胃1周600 mg/kg MOS。结果表明:与空白组相比,ASA1组血清白细胞介素-2(IL-2)含量及溶菌酶(LZM)、总超氧化物歧化酶(T-SOD)活性和肠道隐窝深度(CD)无显著差异(P>0.05),ASA2组血清IL-2含量和CD无显著差异(P>0.05);ASA3组血清T-SOD活性较模型组显著升高(P<0.05),但是与空白组仍存在显著差异(P<0.05),但肠道绒毛高度与隐窝深度比值(VH/CD)较模型组显著升高(P<0.05),且与空白组不存在显著差异(P>0.05)。与空白组相比,模型组黏膜分泌型免疫球蛋白A(sIgA)含量减少了30.32%(P<0.05),ASA1组不存在显著差异(P>0.05),ASA2和ASA3组分别降低了7.39%和12.50%(P<0.05);与模型组相比,ASA3组升高了25.59%(P<0.05)。在本试验条件下,600 mg/kg的MOS对低浓度ASA诱导的大鼠肠道损伤有较好的修复效果,提高了ASA1组大鼠血清中IL-2含量和LZM活性及sIgA含量,改善肠道绒毛结构,提高CD和VH/CD;与模型组相比,MOS促进了ASA3组大鼠肠道发育并提高了血清T-SOD活性。The objective of the experiment was to investigate the therapeutic effect of mannose oligosaccharides(MOS)on repairmen of aspirin(ASA)induced intestinal injury in rats.The experiment was designed by single factor test,36 rats at 6 weeks of age with body weight of 150 to 180 g were randomly divided into 6 groups after 1 week adaptive feeding,each group had 6 rats which fed in a single cage.The model group was administered orally 200 mg/kg ASA for 2 weeks then saline for 1 week.The MOS group was administered orally 600 mg/kg MOS.The ASA1,ASA2 and ASA3 groups were administered orally 50,100 and 200 mg/kg ASA,respectively,then 600 mg/kg MOS for the next week.The blank group received the same amount of normal saline.The results showed that compared with the blank group,there was no significant difference in interleukin-2(IL-2)content,lysozyme(LZM)and total superoxide dismutase(T-SOD)activities and intestinal crypt depth(CD)in ASA1 group(P>0.05),no significant difference in IL-2 content and CD in ASA2 group and control group(P>0.05)and ASA3 group was higher than model group(P<0.05),the ratio of villi height to crypt depth(VH/CD)was higher than that in model group(P<0.05),and there was no difference from the blank group(P>0.05).Compared with blank group,the content of mucosal secretarial immunoglobulin A(sIgA)in model group was decreased by 30.32%(P<0.05),but there was no significant difference from ASA1 group(P>0.05),and it was decreased by 7.39%and 12.50%in ASA2 and ASA3 groups,respectively(P<0.05).Compared with model group,it was increased by 25.59%in ASA3 group(P<0.05).The present study is demonstrated that 600 mg/kg MOS administered orally to the rats with intestinal injury which induced by low concentration of ASA can improve the rats intestinal morphology,increase intestinal CD and VH/CD,and increase the serum IL-2 content and LZM activity and sIgA content in rats in ASA1 group,MOS can promote intestinal development and increase serum T-SOD activity in the ASA3 group compared with the model group.

关 键 词：甘露寡糖 阿司匹林 肠道损伤 肠道修复 

分 类 号：S811.3[农业科学—畜牧学]