饲粮中锌添加水平对生长獭兔毛囊发育及相关基因表达的影响  

Effects of Dietary Zinc Supplemental Level on Hair Follicle Development and Related Gene Expression of Growing Rex Rabbits

作　　者：赵曼 杨国雨 刘永需 刘磊 李福昌 ZHAO Man;YANG Guoyu;LIU Yongxu;LIU Lei;LI Fuchang(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Qingdao Kangda Food Co.,Ltd.,Qingdao 266555,China)

机构地区：[1]山东农业大学动物科技学院,山东省动物生物工程与疾病防治重点实验室,泰安271018 [2]青岛康大食品有限公司,青岛266555

出　　处：《动物营养学报》2021年第6期3532-3541,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业产业技术体系建设专项(CARS-43-B-1);山东省博士后创新项目(2020年);新疆自治区重点研发计划项目(2020B01004-1-3);泰山产业领军人才项目(TSCY20190107)。

摘　　要：本试验旨在研究饲粮中锌添加水平对生长獭兔毛囊发育及相关基因表达的影响。选择160只初始体重相近的3月龄健康獭兔,随机分为4组,每组40个重复,每个重复1只兔。4组獭兔分别饲喂在基础饲粮中添加0(对照组)、40、80和120 mg/kg锌(以硫酸锌的形式)的饲粮。预试期7 d,正试期49 d。结果显示:与对照组相比,饲粮中添加40和80 mg/kg锌时獭兔的毛囊密度显著升高(P<0.05),饲粮中添加120 mg/kg锌对獭兔的毛囊密度无显著影响(P>0.05)。与对照组相比,饲粮中添加40和80 mg/kg锌显著提高了獭兔神经诱导蛋白(Noggin)基因的相对表达量(P<0.05),显著降低了骨形态发生蛋白4(BMP4)基因的相对表达量(P<0.05),对胞外基质蛋白多糖(Versican)、碱性磷酸酶(ALP)、肝细胞生长因子(HGF)、骨形态发生蛋白2(BMP2)和胰岛素样生长因子-1(IGF-1)基因的相对表达量没有显著影响(P>0.05)。综上所述,以硫酸锌形式在3~5月龄生长獭兔饲粮中添加40或80 mg/kg锌时,可通过上调Noggin基因的表达,下调BMP4基因的表达,减少对毛囊生长的抑制并激活毛囊上皮细胞增殖,从而促进毛囊的形成和发育。The aim of this study was to investigate the effects of dietary zinc supplemental level on hair follicle development and related gene expression of growing Rex rabbits.One hundred and sixty 3-month-old Rex rabbits with similar body weight were selected and randomly divided into 4 groups with 40 replicates in each group and 1 rabbit in each replicate.Rex rabbits in the 4 groups were fed diets supplemented with 0(control group),40,80 and 120 mg/kg zinc(in the form of zinc sulfate),respectively.The pre-experimental period lasted for 7 days,and the experimental period lasted for 49 days.The results showed as follows:compared with the control group,diets supplemented with 40 and 80 mg/kg zinc could significantly increase the hair follicle density of Rex rabbits(P<0.05),but diets supplemented with 120 mg/kg zinc had no significant influence on the hair follicle density(P>0.05).Compared with the control group,diets supplemented with 40 and 80 mg/kg zinc could significantly increase the relative expression level of noggin protein(Noggin)gene of Rex rabbits(P<0.05),but significantly decrease the relative expression level of bone morphogenetic protein 4(BMP4)gene(P<0.05).The relative expression levels of extracellular matrix proteoglycans(Versican),alkaline phosphatase(ALP),hepatocyte growth factor(HGF),bone morphogenetic protein 2(BMP2)and insulin-like growth factor-1(IGF-1)genes were not significantly affected by dietary zinc supplemental level(P>0.05).In summary,adding 40 or 80 mg/kg zinc in the form of zinc sulfate to the diet of 3-to 5-month-old growing Rex rabbits can promote the formation and development of hair follicles by up-regulating the expression of Noggin gene and down-regulating the expression of BMP4 gene,reduce the inhibitory effect on hair follicle growth and activate the proliferation of hair follicle epithelial cells,and then promote the formation and development of hair follicle.

关 键 词：獭兔 锌 毛囊发育 基因表达 

分 类 号：S816[农业科学—饲料科学]