分子标记辅助聚合多抗番茄砧木材料的筛选  

Screening of Tomato Rootstock with Multi-resistance by Molecular Marker-assisted Selection

作　　者：杜文丽[1] 林文[1] 钟开勤[1] 陈继兵[1] 陈中钐 高山[1] DU Wen-li;LIN Wen;ZHONG Kai-qin;CHEN Ji-bing;CHEN Zhong-shan;GAO Shan(Fuzhou Institute of Vegetable Crops,Fuzhou 350111,China)

机构地区：[1]福州市蔬菜科学研究所,福建福州350111

出　　处：《江西农业学报》2021年第6期7-12,共6页Acta Agriculturae Jiangxi

基　　金：福州市科技项目“抗病砧木番茄新品种选育”(2018-N-2)。

摘　　要：以20份高世代稳定番茄砧木自交系为材料,对番茄砧木材料进行田间主要病害抗病性评价,以期筛选出抗性材料,为抗病育种提供材料。通过田间抗性鉴定和对5种病害抗性基因(I-2、RRS-342、Tm-2a、Mi-1、Cf-9)进行检测,进而鉴定筛选出聚合多抗番茄砧木。结果表明,Y-7-1、ZM22-1-10的青枯病、根线虫、烟草花叶病毒、叶霉病病情指数均达到抗病以上水平,且均检测到所有的抗病基因标记,是聚合多抗番茄砧木材料。In this study,20 stable tomato rootstocks from high-generation inbred lines were used to evaluate field disease resistance,so as to screen out resistant materials for breeding.By resistance identification in the field and resistance genes testing(RRS-342,I-2,Mi-1,Tm-2a and Cf-9),tomato rootstocks pyramiding multi-resistant genes could be identified.The results showed that Y-7-1 and ZM22-1-10 had high resistance to bacterial wilt,root-knot nematode,tobacco mosaic and cladosporium fulvum,and had all multi-resistant genes by molecular marker.Therefore,Y-7-1 and ZM22-1-10 were tomato rootstock materials with multi-resistance.

关 键 词：番茄砧木 田间抗性鉴定 分子标记 聚合多抗材料 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]