Regulatory Role of Retinoic Acid in Male Pregnancy of the Seahorse  

作　　者：Chunyan Li Yongxin Li Geng Qin Zelin Chen Meng Qu Bo Zhang Xue Han Xin Wang Pei-yuan Qian Qiang Lin 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,South China Sea Institute of Oceanology,Innovation of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences,510301 Guangzhou,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),511458 Guangzhou,China [3]Department of Chemistry,The University of Hong Kong,Pokfulam,Hong Kong,Hong Kong SAR,China [4]Department of Ocean Science and Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Hong Kong University of Science and Technology,Kowloon,Hong Kong,China

出　　处：《The Innovation》2020年第3期72-80,共9页创新（英文）

基　　金：supported by the National Natural Science Foundation of China(41825013,41806189),China;the K.C.Wong Education Foundation,China;the Key Special Project for Introduced Talents Teamof SouthernMarine Science and EngineeringGuangdong Laboratory(Guangzhou)(GML2019ZD0407),China;the Guangdong Special Support Program of Youth Scientific and Technological Innovation(2017TQ04Z269),China;the ChinaPostdoctoral Science Foundation grant(2019M663151),China.

摘　　要：Seahorses epitomize the exuberance of evolution.They have the unique characteristic of male pregnancy,which includes the carrying of many embryos in a brood pouch that incubates and nourishes the embryos,similar to the mammalian placenta.However,the regulatory networks underlying brood pouch formation and pregnancy remain largely unknown.In this study,comparative transcriptomic and metabolomic profiling on the lined seahorse Hippocampus erectus,with unformed,newly formed,and pregnant brood pouches identified a total of 141 and 2,533 differentially expressed genes together with 73 and 121 significantly differential metabolites related to brood pouch formation and pregnancy,respectively.Specifically,integrative omics analysis revealed that retinoic acid(RA)synthesis and signaling pathway played essential roles in the formation of the brood pouch and pregnancy.RA might function upstream of testosterone and progesterone,thereby directly influencing brood pouch formation by regulating the expression of fshr and cyp7a1.Our results also revealed that RA regulates antioxidant defenses,particularly during male pregnancy.Alternatively,pregnancy caused a consistent decrease in RA,canthaxanthin,astaxanthin,and glutathione synthetase,and an increase in susceptibility to oxidative stress,which may balance brood pouch development and reproduction in seahorses and pave the way to successful gestation.

关 键 词：SEAHORSE MALE PREGNANCY BROOD POUCH INTEGRATIVE OMICS RETINOIC ACID ANTIOXIDANT DEFENSE 

分 类 号：R71[医药卫生—妇产科学]