3D打印HA微球支架的制备与表征  

3D Printing and Characterization of Microsphere Hydroxyapatite Scaffolds

作　　者：吴重草 郇志广[2,3] 朱钰方 吴成铁[2,3] WU Zhongcao;HUAN Zhiguang;ZHU Yufang;WU Chengtie(School of Materials Science and Engineering,Shanghai University,Shanghai 200444,China;State Key Lab of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]上海大学材料科学与工程学院,上海200444 [2]中国科学院上海硅酸盐研究所,高性能陶瓷与超微结构国家重点实验室,上海200050 [3]中国科学院大学材料科学与光电工程研究中心,北京100049

出　　处：《无机材料学报》2021年第6期601-607,共7页Journal of Inorganic Materials

基　　金：国家自然科学基金(51761135103)。

摘　　要：生物材料表面微结构对于成骨具有重要的影响,该研究以不同粒径(<60μm)的羟基磷灰石(HA)微球状粉体为原料,通过3D打印技术制备了一系列(HA0、HA10、HA30、HA50)生物陶瓷支架。不同支架具有相似的理化性能,由于微球粒径不同形成了不同的微结构,对其生物学性能造成不同的影响。相比传统非微球颗粒打印的支架(HA0),HA微球构成的支架能够提供更多细胞粘附和生长位点,24 h的粘附实验显示HA30支架能显著促进骨髓间充质干细胞的伪足伸长;培养5 d的细胞增殖实验显示,微球支架上的细胞数量与HA0支架出现显著性差异,表面微球结构与细胞尺度相当的HA30支架具有最好的促增殖效果。因此,3D打印技术在可控制备HA支架宏观结构的同时,还可以通过控制生物陶瓷粉体的颗粒形貌,调控3D打印支架的表面微结构,从而优化其生物学效应,在骨组织工程领域具有良好的应用前景。Surface microstructure of biomaterials plays an important role in osteogenesis.In this study,we prepared a series of hydroxyapatite(HA)scaffolds(HA0,HA10,HA30,HA50)by 3D printing microspheres with different particle sizes(<60μm).These scaffolds have similar physical and chemical properties,but form different microstructures due to their different particle size of microspheres,which result in certain impact on their biological properties.Compared with the traditional scaffolds without micro-sphere structure(HA0),the microsphere-based hydroxyapatite scaffolds provide improved adhesion and growth sites for bone mesenchymal stem cells(BMSCs)after 24 h culture,among which HA30 scaffold significantly promotes the pseudopodia elongation of BMSCs.After 5 d of cell culture on the scaffolds,the micro-sphere based HA scaffolds significantly stimulate proliferation of the BMSCs in contrast to the HA0 group.HA30 scaffolds with microsphere at similar size to the cell size have the best promoting effect on cell proliferation.Therefore,3D printing technology can not only control the macrostructure of HA scaffolds,but also construct the microstructure on the surface of HA scaffolds by controlling the particle sizes of bioceramic powders to optimize the biological effects,which shows great potential application in the field of bone tissue engineering.

关 键 词：HA微球 3D打印 支架 骨组织工程 

分 类 号：TQ174[化学工程—陶瓷工业]