间充质干细胞与成纤维细胞生物学特性的比较及研究进展  

The Comparative Study on the Characterization of Mesenchymal Stem Cells and Fibroblasts

作　　者：赵强 白海 苏毅 姚浩 ZHAO Qiang;BAI Hai;SU Yi;YAO Hao(Department of Hematology,the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army,Lanzhou 730050,China;Department of Hematology,the General Hospital of Western Theater Command of Chinese People’s Liberation Army,Chengdu 610083,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院血液科,兰州730050 [2]中国人民解放军西部战区总医院血液科,成都610083

出　　处：《中国细胞生物学学报》2021年第5期1068-1074,共7页Chinese Journal of Cell Biology

基　　金：西部战区总医院学科助推基金(批准号:41732E7);甘肃省科技计划项目(批准号:20JR5RA602)资助的课题。

摘　　要：间充质干细胞(mesenchymal stem cells,MSCs)具有多向分化潜能和免疫调节能力,且不具伦理争议的特点使其数十年来在基础和临床研究中得到广泛应用。然而,MSCs在形态上并不能和成纤维细胞有所区分。该文对这两种细胞在生物学特性及临床应用方面的异同点和两者可能存在的关系进行了综述。结果表明:这两种细胞在细胞免疫表型、增殖、分化甚至基因表型和免疫调节能力方面几乎完全一样;不同之处在于,MSCs在上述能力上比成纤维细胞表现得更加高效。目前,并没有特异性的标志可以区分这两种细胞,但两者的甲基化谱略有不同,而两者之间的差异类似于不同代数的MSCs之间的区别,某些细胞表面标志物的表达也表现出类似的现象。按照现行的国际细胞治疗学会对MSCs的定义,成纤维细胞甚至也可以被命名为MSCs。因此,两者可能是同一种细胞,只是成纤维细胞是代数比较高的MSCs。MSCs (mesenchymal stem cells) have the abilities of multi-differentiation and immune regulation,which makes them unique in the research of many adult tissue stem cells for decades.However,MSCs cannot be distinguished from fibroblasts in morphology.The similarities and differences between these two kinds of cells and the possible relationship were reviewed in this article.The results showed that the two kinds of cells were almost identical in cell immunophenotype,proliferation,differentiation and even gene phenotype and immune regulation ability.The difference is that MSCs are more efficient than fibroblasts in above-mentioned abilities.Meanwhile,there are no specific markers to distinguish the two kinds of cells,but their methylation profiles are slightly different.Interestingly,some of the differences between them are similar to that of MSCs with different passages,and the expression of some cell surface markers also show similar phenomenon.According to the current definition of MSCs by the International Society of Cell Therapy,fibroblasts can even be named as MSCs.Therefore,they may be the same cells.However,fibroblasts are aging MSCs.

关 键 词：间充质干细胞 成纤维细胞 免疫调节 细胞表面标志物 基因表达 甲基化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]