广西一个黑髯墓蝠群落的病毒组研究  

Virome of a Black-bearded Tomb Bat Colony in Guangxi,China

作　　者：谈之舟 孟菲[3] 徐琳 涂长春[1,2] 吴健敏 何彪[2] TAN Zhizhou;MENG Fei;XU Lin;TU Changchun;WU Jianmin;HE Biao(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;Institute of Military Veterinary Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Changchun 130122,China;Guangxi Veterinary Research Institute,Nanning 530001,China)

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]军事科学院军事医学研究院军事兽医研究所,长春130122 [3]广西壮族自治区兽医研究所,南宁530001

出　　处：《吉林农业大学学报》2021年第1期109-118,共10页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(31572529)。

摘　　要：蝙蝠是许多重要人兽共患病毒的自然宿主。对广西壮族自治区的一个蝙蝠群落进行了病毒监测,共采集了95只黑髯墓蝠(Taphozous melanopogon),对其肺脏和肠道及其内容物进行了病毒宏基因组学分析。结果表明:测得的病毒序列分属于17个病毒科,其中包括哺乳动物、节肢动物和植物等来源的病毒及噬菌体。进一步对发现的环状病毒(Orbivirus)和Pegivirus进行了特异性PCR检测,其中环状病毒在肺脏中阳性率为10.5%(10/95),肠道阳性率为36.8%(35/95),其中8只蝙蝠2种脏器均为阳性;进一步扩增得到了环状病毒的4个节段的部分序列,对RdRP的多序列比较和系统进化显示,该环状病毒在库蠓和白蛉来源环状病毒群和蚊类来源环状病毒群之间形成了一个独特的进化分支,与已知环状病毒的氨基酸相似度仅为45%~55%,是一种新型的环状病毒。另外,通过qRT-PCR对Pegivirus进行检测,发现其阳性率为78.9%(75/95),其中病毒滴度最高的为每毫克8.56×10^(4)个病毒拷贝,并在5个肺脏阳性样品中扩增得到了5株Pegivirus,它们之间的核苷酸相似度为98%~99%,而与非洲喀麦隆发现的墓蝠Pegivirus的核苷酸相似度为76%,进化树显示其在Pegivirus G进化群中形成了一个新的进化分支。此研究增加了对黑髯墓蝠病毒组的认识,其中发现的环状病毒和Pegivirus揭示了相关病毒的遗传多样性。Bats are the reservoir hosts of numerous high-consequence pathogenic viruses with epizootic and zoonotic potential. In the present study, we report the findings of viral surveillance of a bat colony located in Guangxi Zhuang Autonomous Region in China. Black-bearded tomb bats(Taphozous melanopogon;n=95) were collected, and their lungs, rectal tissue and fecal contents were sequenced and analyzed by a viral metagenomic pipeline. The results revealed viral sequences of 17 families derived from mammals, arthropods, plants and bacteria. Furthermore, one strain of a novel orbivirus and five strains of a novel pegivirus were detected by virus-specific PCR, respectively. The orbivirus had a 10.5%(10/95) viral RNA positivity rate in lung tissues and 36.8%(35/95) in rectal tissue. Interestingly, eight bats were positive in both lung and rectal tissues. A partial genome sequence was recovered from lung tissue from one bat and partial segments encoding the RdRp, T2, CaP and OC2 structural proteins were obtained. Sequence comparison and phylogenetic analysis of RdRp revealed the orbivirus represents a novel species within the genus Orbivirus, with 45%-55% amino acid identity with previously described orbiviruses, and formed a unique lineage between the Culicoides, Phlebotominae and mosquito-borne orbivirus clades. To determine the viral titer of the pegiviruses, a qRT-PCR showed an extremely high prevalence rate: 78.9%(75/95) positivity in lung tissue with the highest viral titer detected of 8.56×10^(4) copies/mg. Partial genomic sequences were recovered from five pegiviruses positive bat lung samples and exhibited 98%-99% nucleotide sequence identity with one another. The pegivirus exhibited 76% nucleotide sequence identity with the Taphozous bat Pegivirus previously identified in Cameroon, and formed a novel lineage in Pegivirus clade G. The discovery of these novel orbi-and pegiviruses in Taphozous melanopogon extends the existing knowledge of bat viromic diversity.

关 键 词：黑髯墓蝠 病毒宏基因组学 环状病毒 Pegivirus 

分 类 号：S858.99[农业科学—临床兽医学]