昆虫病原线虫JLSY 003的鉴定及其生物学特性测定  

Identification and Biological Characteristics of Entomopathogenic Nematode JLSY 003

作　　者：田成丽 李茂海[1] 刘金文[1] 张金花[1] 朱峰[1] 李建平[1] TIAN Chengli;LI Maohai;LIU Jinwen;ZHANG Jinhua;ZHU Feng;LI Jianping(Institute of Plant Protection,Jilin Academy of Agricultural Sciences,Gongzhuling 136100)

机构地区：[1]吉林省农业科学院植物保护研究所,公主岭136100

出　　处：《中国生物防治学报》2021年第3期480-485,共6页Chinese Journal of Biological Control

基　　金：吉林省农业科技创新工程重大项目(CXGC2017ZD007)。

摘　　要：经28S rDNA和ITS序列分析,从吉林省松原市土样中分离到的昆虫病原线虫JLSY 003为Oscheius myriophila品系。本研究以大蜡螟末龄幼虫为寄主,测定该线虫的生物学特性。结果表明,JLSY 003浓度为80 IJs/larva时具有较高的侵染力,致死中时间LT_(50)为15.409 h,侵染24、48 h时,致死中浓度LC_(50)分别为46.067、25.297 IJs/larva。JLSY 003高温耐受性强且繁殖力高,36℃、8 h时线虫死亡率为19.27%,产量为1.873×10^(5) IJs/larva,4.695×10^(5) IJs/g。结果表明该线虫具有较高的致病力、耐热性和繁殖力。The entomopathogenic nematode was isolated from the soil samples collected in Songyuan,Jilin province,China.It was identified as Oscheius myriophila strain by ITS and 28 S r DNA sequences,and named JLSY003.Last instar of Galleria mellonella was used as host to test JLSY003 biological characteristics.The results showed that it had a high pathogenicity at 80 IJs/larva,LT50 was 15.409 h.LC_(50) was 46.067 and 25.297 IJs/larva at 24 and 48 h,respectively.JLSY 003 had a high temperature tolerance and a high fertility.The nematode mortality rate was 19.27%at 36℃and for 8 h,the yield was 1.873×10^(5) IJs/larva or 4.695×10^(5) IJs/g.The results showed that JLSY003 had a high pathogenicity,a high temperature torlerance and a high fertility.

关 键 词：昆虫病原线虫 Oscheius myriophila LC_(50) LT_(50) 耐热性 

分 类 号：Q965[生物学—昆虫学]