几种茶园杂草生防菌的分离鉴定  

Isolation and Identification of Main Weed Pathogens in Tea Garden

作　　者：陆俞萍 肖婉 房婉萍[2] 强胜[1] 陈世国[1] LU Yuping;XIAO Wan;FANG Wanping;QIANG Sheng;CHEN Shiguo(Weed Research Laboratory,Nanjing Agricultural University,Nanjing 210095,China;Tea Science Research Institute,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学杂草研究室,南京210095 [2]南京农业大学茶叶科学研究所,南京210095

出　　处：《中国生物防治学报》2021年第3期564-574,共11页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0201300);国家现代茶叶产业技术体系(CARS-19);江苏省重点研发计划(BE2019379)。

摘　　要：为发掘具有防治茶园杂草潜力的生防菌,本研究从江苏、浙江和安徽3省共16个茶园采集感病杂草,采用组织分离法分离出病原菌,通过菌饼回接验证获得了44株具有致病力的植物病原菌,从中筛选出了10株致病性较强的优势菌株。利用柯赫氏法则验证优势菌株后,通过观察优势病原菌的形态特征,结合rDNA-ITS基因片段序列分析,确定10株优势菌株分别为平脐蠕孢属的狗尾草平脐蠕孢Bipolaris setariae、链格孢属的链格孢霉Alternariaalternata、弯孢属的间型弯孢Curvulariaintermedia和新月弯孢Curvularialunata、镰刀属的再育镰刀菌Fusariumproliferatum和木贼镰刀菌Fusariumequiseti、棒孢属的多主棒孢Corynesporacassiicola和茎点霉属的荸荠茎点霉Didymellabellidis,共6属8种的病原真菌。结合作物安全性问题,木贼镰刀菌、再育镰刀菌、狗尾草平脐蠕孢、间型弯孢和新月弯孢5种病原菌被认为具有开发成防除茶园杂草生物除草剂的较好潜力。To screen out plant pathogens with strong pathogenicity to weeds and high safety to tea plant,the diseased weeds were collected from 16 tea gardens in Jiangsu,Zhejiang and Anhui provinces.Forty-four plant pathogens were isolated,thereinto,10 fungus strains with high pathogenicity were selected and further tested by Koch’s postulates.Based on morphological characteristics and sequence analysis of rDNA-ITS genes,these ten stains were identified as Bipolaris setariae,Alternaria alternata,Curvularia intermedia and C.lunata,Fusarium proliferatum and F.equiseti,Corynespora cassiicola and Didymella bellidis,belonging to 8 different species of pathogenic fungi.Considering crop safety,five candidate strains,F.equiseti,F.proliferatum,B.setariae,C.intermedia and C.lunata,exhibit the good potential for development into bioherbicides in weed control in tea garden.

关 键 词：茶 病原菌 分离与鉴定 生物除草剂 

分 类 号：S476[农业科学—农业昆虫与害虫防治]