Let-7d*在增生性血管疾病中的表达及其作用机制  

Expression of Let-7d*and its role in proliferative vascular diseases

作　　者：邵帅 余烊[1] 秦姗姗 刘远辉[1] 谭宁[1] SHAO Shuai;YU Yang;QIN Shan-shan;LIU Yuan-hui;TAN Ning(Department of Cardiology,Guangdong Cardiovascular Institute,Guangdong Provincial Key Laboratory of Coro-nary Heart Disease Research,Guangdong Provincial People′s Hospital,Guangdong Academy of Medical Scienc-es,Guangzhou 510080,China)

机构地区：[1]广东省心血管病研究所心内科、广东省冠心病防治研究重点实验室、广东省人民医院(广东省医学科学院),广州510080

出　　处：《岭南心血管病杂志》2021年第3期337-343,共7页South China Journal of Cardiovascular Diseases

基　　金：国家自然科学基金(项目编号:81670334)。

摘　　要：目的探究Let-7d*在增生性血管疾病中的表达及其相关作用机制。方法分别构建球囊损伤模型与平滑肌细胞增殖模型,研究Let-7d*在这两种模型中的表达趋势,并调控Let-7d*的表达,研究其相关生物学功能。结果在球囊损伤诱导新生内膜生长的模型中,Let-7d*的表达水平较在正常颈动脉中明显降低,同时在增殖的血管平滑肌细胞中的表达也明显下调。在体内动物模型中Let-7d*对球囊损伤大鼠颈动脉血管内膜的增殖具有抑制作用,在体外细胞模型中Let-7d*是血管平滑肌细胞生长的一个强有力的负性调控因子。结论Let-7d*在增殖的血管平滑肌细胞和内膜增生的血管壁中的表达下调,且Let-7d*对血管平滑肌细胞生长及大鼠颈动脉新生内膜增生均有明显的抑制作用。Objectives To investigate the expression of Let-7d*in proliferative vascular diseases and its mechanism.Methods A balloon injury model was constructed in vivo and a smooth muscle cell proliferation model was constructed in vitro to study the trend of Let-7d*in these two models,and to regulate the expression of Let-7d*and observe its related effects.Results The expression of Let-7d*in rat carotid arteries induced by balloon injury neointimal growth was significantly lower than that in normal arteries,and the expression of Let-7d*in proliferating vascular smooth muscle cells was also significantly reduced.In vivo animal model,Let-7d*had an inhibitory effect on the proliferation of carotid artery intima in balloon injured rats.In vitro cell model,Let-7d*was a powerful negative regulator of vascular smooth muscle cell growth.Conclusions Expressions of Let-7d*in the balloon injury model in vivo and the smooth muscle cells proliferation model in vitro reduce,and Let-7d*inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia of carotid artery in rats.

关 键 词：增生性血管疾病 平滑肌细胞 微小RNA 球囊损伤模型 Let-7d 

分 类 号：R33[医药卫生—人体生理学]