荧光聚多巴胺-金量子点应用于癌细胞的高效成像检测  

High efficiency imaging detection of cancer cells by fluorescent polydopamine-gold dots

作　　者：周瑜 谭虹 邓谦亦 左芳[2] ZHOU Yu;TAN Hong;DENG Qianyi;ZUO Fang(Institute of Qinghai-Tibet Plateau,Southwest Minzu University,Chengdu 610041,China;School of Chemistry&Environment,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学青藏高原研究院,四川成都610041 [2]西南民族大学化学与环境学院,四川成都610041

出　　处：《广西科技大学学报》2021年第3期102-107,共6页Journal of Guangxi University of Science and Technology

基　　金：中央高校基本科研业务费专项基金项目(2020NZD01)资助.

摘　　要：为了准确识别癌细胞,制备了一种具有荧光性能的聚多巴胺-金量子点.该量子点纳米材料是基于前人方法,在室温下以多巴胺为唯一前体控制合成来制备小尺寸聚多巴胺,然后在高温、过氧化氢存在的条件下利用产生的羟基自由基去破坏聚多巴胺的π-π结构来生成具有荧光性能的聚多巴胺量子点,并在其表面通过还原氯金酸形成聚多巴胺-金量子点;利用荧光成像技术,通过观察聚多巴胺-金量子点的荧光淬灭情况来实现对癌细胞中谷胱甘肽(GSH)的检测.结果表明,该量子点纳米材料可以实现对癌细胞的特异性荧光成像检测,为开发高效的识别生物传感器和对癌细胞的精准识别提供了一种有效的策略.Akind of fluorescent polydopamine-glod dots were prepared to identify cancer cells accurately.Based on the previous method,small size polydopamine was synthesized at room temperature with dopamine as the only precursor,then,the hydroxyl radical was used to destroy the π-π structure of polydopamine at high temperature in the presence of hydrogen peroxide,which can be used to generate polydopamine dots with fluorescence properties.Finally,chloroauric acid was reduced on its surface to form polydopamine-glod dots.Glutathione(GSH)in cancer cells was detected by fluorescence imaging by observing the fluorescence quenching of polydopamine-gold quantum dots.The results show that the quantum dot nanomaterials can realize the specific fluorescence imaging detection of cancer cells,which provides an effective strategy for developing efficient recognition biosensors for cancer cells and identifying cancer cells accurately.

关 键 词：癌细胞 聚多巴胺-金 谷胱甘肽 荧光成像 纳米材料 

分 类 号：TB383[一般工业技术—材料科学与工程] O657.3[理学—分析化学]