赤芝规模化液态深层发酵及其产物抗肿瘤活性研究  

Large scale submerged fermentation of Ganoderma lucidum and antitumor activity of its product

作　　者：朱晓璐 韩伟 冯娜[1] 唐庆九[1] 袁峰 徐国华 冯杰[1] 张劲松[1] ZHU Xiaolu;HAN Wei;FENG Na;TANG Qingjiu;YUAN Feng;XU Guohua;FENG Jie;ZHANG Jingsong(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,P.R.China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,School of Pharmacy,East China University of Science and Technology,Shanghai Key Laboratory of New Drug Design,Shanghai 200237,China;Jiangsu Shenhua Pharmaceutical Co.Ltd.,Medicinal Fungi Biotechnology Research Center of Jiangsu Province,Huai’an 211600,China)

机构地区：[1]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海201403 [2]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海200237 [3]江苏神华药业有限公司,江苏省药用真菌生物工程技术研究中心,淮安211600

出　　处：《上海农业学报》2021年第3期24-28,共5页Acta Agriculturae Shanghai

基　　金：国家自然科学基金青年科学基金项目(31801919);上海市科技兴农重点攻关项目[沪农科创字(2018)第1-1号];上海人才发展资金项目(2019052)。

摘　　要：利用1 000 L工业级别发酵罐规模化制备赤芝液态深层发酵活性产物,获得的30 L发酵产物经醇提浓缩后用石油醚、乙酸乙酯依次萃取,得到3种不同极性部位的萃取物,分别评价其抗肿瘤活性。结果表明:发酵过程中添加油酸可以促进活性产物的合成,发酵产物中三萜及甾醇含量高达8.91 mg∕(100 mg干菌丝);石油醚萃取物与乙酸乙酯萃取物均表现出良好的抗L1210和K562肿瘤细胞活性。可见,液态深层发酵技术是规模化制备赤芝活性物质的有效手段,可为后续活性成分分离纯化的研究提供稳定的材料来源。The 30 L fermentation product of Ganoderma lucidum was prepared by large-scale production of 1000 L industrial grade fermentor.After ethanol extraction and concentration,the fermentation product was extracted with petroleum ether and ethyl acetate in turn to obtain three extracts with different polarity,and their antitumor activities were evaluated respectively.The results showed that the addition of oleic acid could promote the synthesis of active product,and the content of triterpenoids and sterols in fermentation product was up to 8.91 mg∕(100 mg dry mycelium);Petroleum ether extract and ethyl acetate extract showed good anti-L1210 and K562 tumor activity.It can be seen that submerged fermentation technology is an effective method for large-scale preparation of active substances of G.lucidum,which can provide a stable material source for the subsequent study of separation and purification of active ingredients.

关 键 词：灵芝 液态深层发酵 规模化制备 抗肿瘤活性 

分 类 号：S567.3[农业科学—中草药栽培]