异丙酚对脓毒症小鼠中性粒细胞胞外诱捕网生成和炎症因子表达的影响  

Effects of Propofol on production of neutrophil extracellular traps and expression of inflammatory cytokines in sepsis mice

作　　者：杨鹤[1] 郑莉[1] 张晔[1] 佟家祺 岳郅劼 YANG He;ZHENG Li;ZHANG Ye(Department of Anesthesiology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院麻醉科,北京100050

出　　处：《临床和实验医学杂志》2021年第11期1135-1138,共4页Journal of Clinical and Experimental Medicine

基　　金：首都医科大学科研培育基金(校自然)项目(编号:PYZ2018014);北京市医管局培育项目(编号:PZ20190102);首都医科大学附属友谊医院院启动项目(编号:YYQDKT2015-7)。

摘　　要：目的明确异丙酚对脓毒症小鼠模型中性粒细胞胞外诱捕网(NETs)生成和炎症因子表达的影响。方法雄性8~10周龄C57BL/6小鼠40只,按照随机数字表法分为对照组、脂多糖(LPS)组、异丙酚组和脱氧核糖核酸酶(DNase)组,每组各10只。腹腔注射LPS建立脓毒症模型;异丙酚组小鼠腹腔注射异丙酚全身麻醉1 h;DNase组腹腔注射DNase维持1 h;对照组腹腔注射等量的0.9%氯化钠溶液,观察相同时间。PicoGreen荧光染料法检测4组小鼠血浆游离DNA(cf-DNA/NETs)水平;酶联免疫吸附试验测定Toll样受体-4(TLR-4)、肿瘤坏死因子-α(TNF-α)和髓过氧化物酶(MPO)水平。结果LPS组小鼠的cf-DNA/NETs、TLR-4、TNF-α和MPO水平分别为(921.23±27.14)ng/mL、(13.88±1.12)ng/mL、(10253.14±199.25)pg/mL、(135.67±9.87)milliunits/mL,均明显高于对照组[(166.54±11.83)、(2.21±0.29)ng/mL、(2924.68±25.53)pg/mL、(32.83±1.47)milliunits/mL],差异均有统计学意义(P<0.05);异丙酚组小鼠的cf-DNA/NETs、TLR-4、TNF-α和MPO水平[(611.55±35.17)ng/mL、(9.97±0.51)ng/mL、(6505.03±79.76)pg/mL、(87.77±6.06)milliunits/mL]较LPS组明显降低,差异均有统计学意义(P<0.05);DNase组小鼠的TLR-4、TNF-α水平[(5.39±0.28)ng/mL、(4702.74±114.21)pg/mL]高于对照组,差异均有统计学意义(P<0.05);DNase组小鼠的cf-DNA/NETs、MPO水平[(203.52±7.01)ng/mL,(41.89±3.84)milliunits/mL]与对照组相比,差异均无统计学意义(P>0.05)。结论异丙酚对脓毒症小鼠模型NETs的生成和炎症因子表达具有一定的抑制作用,可适用于脓毒症患者的临床麻醉。Objective To investigate the effect of Propofol on production of neutrophil extracellular traps(NETs)and expression of inflammatory cytokines in sepsis mice.Methods Forty male C57BL/6 mice aged 8-10 weeks were randomly divided into control group,lipopolysaccharide(LPS)group,Propofol group and deoxyribonuclease(DNase)group,10 cases in each group.Sepsis model was established by intraperitoneal injection of LPS;the Propofol proup were given general anesthesia with intraperitoneal injection propofol for 1 hour;the DNase group were intraperitoneal injected DNase,then maintain for 1 hour,the control group was injected with the same amount of physiological saline maintained the same time.PicoGreen fluorescent dyes was used to detect plasma circulating free DNA cf-DNA/NETs concentration;and enzyme linked immunosorbent assay(ELISA)was used to assess the concentration of Toll like receptor-4(TLR-4),tumor necrosis factor-α(TNF-α)and myeloperoxidase(MPO)in 4 groups.Results The levels of cf-DNA/NETs,TLR-4,TNF-αand MPO in the LPS group were(921.23±27.14)ng/mL,(13.88±1.12)ng/mL,(10253.14±199.25)pg/mL,(135.67±9.87)milliunits/mL,which were significantly higher than those in the control group[(166.54±11.83),(2.21±0.29)ng/mL,(2924.68±25.53)pg/mL,(32.83±1.47)milliunits/mL],the differences were statistically significant(P<0.05);the levels of cf-DNA/NETs,TLR-4,TNF-αand MPO in the propofol group[(611.55±35.17)ng/mL,(9.97±0.51)ng/mL,(6505.03±79.76)pg/mL,(87.77±6.06)milliunits/mL]were significantly lower than those in the LPS group,and the difference was statistically significant(P<0.05);the levels of TLR-4 and TNF-αin the DNase group[(5.39±0.28)ng/mL,(4702.74±114.21)pg/mL]were higher than those in the control group,the differences were statistically significant(P<0.05);Compared with the control group,the cf-DNA/NETs and MPO levels of mice in the DNase group[(203.52±7.01)ng/mL,(41.89±3.84)milliunits/mL],the differences were not statistically significant(P>0.05).Conclusion Propofol can inhibit the production of NETs a

关 键 词：小鼠 脓毒症 异丙酚 中性粒细胞胞外诱捕网 炎症因子 

分 类 号：R614[医药卫生—麻醉学]