2017-2019年度上海市松江区A(H1N1)pdm09流感病毒HA和NA基因特征分析  

Hemagglutinin and neuraminidase genetic characteristics of influenza A(H1N1)pdm09 virus in Songjiang,Shanghai,2017-2019

作　　者：吴佳瑾 刘玲 赵雪 盛峰松[1] 乔雪飞[1] Wu Jiajin;Liu Ling;Zhao Xue;Sheng Fengsong;Qiao Xuefei(Songjiang District Center for Disease Control and Prevention,Shanghai 201620,China;Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市松江区疾病预防控制中心,上海201620 [2]上海市疾病预防控制中心,上海200336

出　　处：《疾病监测》2021年第5期423-430,共8页Disease Surveillance

基　　金：上海市卫健委青年项目(No.20174Y0087)。

摘　　要：目的分析上海市松江区2017-2019监测年度A(H1N1)pdm09流感病毒HA和NA基因特征。方法对松江区分离的毒株随机挑选46株A(H1N1)pdm09流感病毒毒株进行HA和NA基因序列进化分析和氨基酸位点变异分析,采用神经氨酸酶抑制实验开展奥司他韦和扎那米韦敏感性监测。结果A(H1N1)pdm09流感病毒属于6B.1A分支且进化出了6B.1A2、6B.1A5A和6B.1A6进化簇。核苷酸序列分析显示,HA和NA基因与WHO推荐的2019-2020年度的疫苗株A/Brisbane/02/2018的亲缘关系较为接近,同源性分别为98.6%~99.4%和99.1%~99.7%。氨基酸序列分析显示,HA1蛋白共有23个位点发生氨基酸序列变异,43.48%病毒发生位于抗原决定簇Cb、Sa和Sb区的S74R、S164T、T185I共同突变,提示病毒发生抗原漂移。鸡胚适应性分析发现HA蛋白D187V、Q223R和E224K位点的适应性突变。NA蛋白共有25个位点发生变异,存在酶活性中心E119K(2/46)突变。神经氨酸酶抑制实验显示,所测流感病毒对奥司他韦和扎那米韦均敏感。结论A(H1N1)pdm09流感病毒HA和NA基因及其编码的氨基酸持续变异,因此应持续监测流感病毒,为科学防控提供依据。Objective To understand the hemagglutinin and neuraminidase genetic variation of influenza A(H1N1)pdm09 virus in Songjiang,Shanghai,during 2017-2019.Methods A total of 46 strains of influenza A(H1N1)pdm09 virus were randomly selected for hemagglutinin and neuraminidase sequencing analysis.Neuraminidase inhibition test was performed to analyze the susceptibilities of the influenza virus strains to Oseltamivir and Zanamivir.Results In the strains of influenza A(H1N1)pdm09 virus,46 belonged to 6 B.1A branch.Most of the virus strains had evolved into 6 B.1 A2,6 B.1 A5 A and 6 B.1 A6 subclades.The homologies of HA and NA nucleotide sequences between the strains and the WHO 2019/20 vaccine strain A/Brisbane/02/2018 were 98.6%-99.4%and 99.1%-99.7%respectively.Amino acid mutations occurred in 23 loci of HA1 protein.S74 R+S164 T+T185 I HA covariation occurred in 43.48%of the strains,indicating the antigenic drift of the influenza virus.Egg-adaptability analysis founded three amino acid changes in the HA1 gene:D187 V,Q223 R and E224 K.25 amino acid mutations occurred in the NA protein,of which E119 K NA mutation occurred in the NA enzyme active site.In neuraminidase inhibition test,all the strains were sensitive to Oseltamivir and Zanamivir.Conclusion The genetic variation existed in the HA and NA gene of influenza A(H1N1)pdm09 virus.It is necessary to strengthen the genetic evolution surveillance for influenza viruses to provide the evidence for the effective prevention and control of influenza.

关 键 词：A(H1N1)pdm09流感病毒 血凝素 神经氨酸酶 基因特征 鸡胚适应性分析 

分 类 号：R211[医药卫生—中医学] R373.13