WEE1 G2检查点激酶对子宫内膜癌细胞增殖、迁移及侵袭的影响  

Effect of WEE1 G2 checkpoint kinase on the proliferation,migration and invasion of endometrial carcinoma cells

作　　者：史丽[1] 马静[1] 赵喜娃[1] 关英霞 赵连梅[2] 单保恩[2] SHI Li;MA Jing;ZHAO Xi-wa;GUAN Ying-xia;ZHAO Lian-mei;SHAN Bao-en(Department of Obstetrics and Gynecology,the Forth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei Province,China;Scientific Research Center,the Forth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei Province,China)

机构地区：[1]河北医科大学第四医院妇产科,河北石家庄050011 [2]河北医科大学第四医院科研中心,河北石家庄050011

出　　处：《中国临床药理学杂志》2021年第12期1539-1542,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省医学科学研究重点课题计划基金资助项目(20201089)。

摘　　要：目的研究WEE1 G2检查点激酶(WEE1)对子宫内膜癌(EC)细胞增殖、迁移以及侵袭的影响及失调机制。方法取RL95-2细胞系培养,并分别转染WEE1小干扰RNA(si-WEE1组)、NEAT1小干扰RNA(si-NEAT1组)、WEE1过表达质粒(pc-WEE1组)、NEAT1过表达质粒(pc-NEAT1组)、miR-139-5p模拟物(miR-139-5p mimic组)、miR-139-5p抑制剂(miR-139-5p inhibitor组),并设立相应对照组(si-NC、pc-NC以及NC mimic)。以实时定量聚合酶链反应(qRT-PCR)检测子宫内膜癌组织和细胞中WEE1 mRNA表达水平,以CCK-8实验检测细胞增殖,以Transwell实验检测细胞迁移和侵袭,以Western blot检测WEE1表达。结果子宫内膜癌患者癌组织和对应癌旁组织中WEE1的表达量分别为1.19±0.21和1.01±0.20;EC细胞系(RL95-2)和人正常子宫内膜细胞(h EEC)中WEE1的表达量分别为1.95±0.15和1.00±0.10;si-NC组和si-WEE1组48 h时细胞光密度值分别为0.68±0.05和0.50±0.02;72 h时为1.12±0.09和0.75±0.05;细胞侵袭数目分别为109.33±8.06和67.67±5.25。癌组织与癌旁组织相比,EC细胞系与人正常子宫内膜细胞系相比,si-WEE1组与si-NC组相比,差异均有统计学意义(均P<0.05)。结论在子宫内膜癌中,NEAT1通过miR-139-5p/WEE1促进EC恶性进展,这可能成为子宫内膜癌的诊断性生物标志物和治疗靶点。Objective To investigate the effect of WEE1 G2 checkpoint kinase(WEE1)on the proliferation,migration and invasion of endometrial carcinoma(EC)cells and its dysregulation mechanism.Methods RL95-2 cell lines were cultured and transfected with WEE1siRNA(si-wee1 group),NEAT1 siRNA(si-NEAT1 group),WEE1overexpression plasmid(pc-WEE1 group),NEAT1 overexpression plasmid(pc-NEAT1 group),miR-139-5p mimic(miR-139-5p mimic group),miR-139-5p inhibitor(miR-139-5p inhibitor group),respectively,and corresponding control groups(si-NC,pcNC and NC mimic)were established.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of WEE1 mRNA in EC tissues and cells,CCK-8 assay was used to detect cell proliferation,and Transwell assay was used to detect cell migration and invasion.Results The expression of WEE1 in the cancer tissue and the corresponding adjacent tissues of patients with endometrial cancer were 1.19±0.21 and 1.01±0.20,the expression levels of WEE1 in EC cell line(RL95-2)and human normal endometrial cells(h EEC)were 1.95±0.15 and 1.00±0.10,the cell absorbance values of si-NC group and si-WEE1 group at 48 h were 0.68±0.05 and 0.50±0.02,;the cell absorbance values of si-NC group and si-WEE1 group at 72 h were 1.12±0.09 and 0.75±0.05,the number of invasive cells in si-NC group and si-WEE1 group were 109.33±8.06 and 67.67±5.25,cancer tissue compared with adjacent tissue,EC cell lines compared with human normal endometrial cell lines,and si-WEE1 group compared with si-NC group,the differences were statistically significant(all P<0.05).Conclusion In endometrial cancer,lncRNA NEAT1 promotes EC malignant progression via miR-139-5p/WEE1,which may serve as a diagnostic biomarker and therapeutic target for EC.

关 键 词：WEE1 G2检查点激酶 长链非编码RNA-核富集转录本1 微小RNA-139-5p 子宫内膜癌 

分 类 号：R97[医药卫生—药品]