当归拈痛汤治疗急性痛风性关节炎疗效和作用机制的小鼠实验研究  

Efficacy and Mechanism of Action of Danggui Niantong Decoction against Acute Gouty Arthritis in a Mouse Model:an Experimental Study

作　　者：陈绍华 王沁筠[2,4,5] 吴昌桂 赵啸 徐浩 施杞[1,2,4] 梁倩倩 CHEN Shaohua;WANG Chinyun;WU Changgui;ZHAO Xiao;XU Hao;SHI Qi;LIANG Qianqian(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Institute of Spinal Diseases,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Shanghai Changning Tianshan Traditional Chinese Medicine Hospital,Shanghai 200051,China;Key Laboratory of Ministry of Education for Musculoskeletal Theory and Treatment,Shanghai 200120,China;School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China)

机构地区：[1]上海中医药大学附属龙华医院,上海市200032 [2]上海中医药大学脊柱病研究所,上海市200032 [3]上海市长宁区天山中医医院,200051 [4]上海筋骨理论与治法教育部重点实验室,上海市200120 [5]上海中医药大学康复医学院,上海市201203 [6]上海交通大学医学院附属瑞金医院,上海市200025

出　　处：《中国全科医学》2021年第24期3116-3121,3128,共7页Chinese General Practice

基　　金：国家自然科学基金优秀青年科学基金项目(81822050)——中医药防治关节炎的应用基础研究。

摘　　要：背景急性痛风性关节炎(AGA)是多因素作用的结果,中药复方以多成分、多靶点作用而在降尿酸、缓解AGA症状等方面具有独特疗效,但具体作用机制尚不完全明确。目的探讨当归拈痛汤治疗AGA的疗效和作用机制。方法本实验于2020-08-17至2020-10-07实施。适应性饲养1周后将30只10~12周龄SPF级雄性C57BL/6小鼠编号并采用随机数字表法分为对照组、溶剂组和DGNTT组,每组10只。对照组小鼠在双侧足掌深层跖跗关节周围注射磷酸盐缓冲液,溶剂组和DGNTT组小鼠在双侧足掌深层跖跗关节周围注射3%尿酸单钠悬液诱导造模;造模完成后对照组和溶剂组小鼠予以0.9%氯化钠溶液灌胃1周,DGNTT组小鼠予以当归拈痛汤中药液灌胃1周。比较三组小鼠治疗前后体质量,造模前、造模后6 h、治疗后1~7 d足掌厚度增长百分比,治疗后7 d注射足部组织白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、诱导型一氧化氮合酶(iNOS)mRNA相对表达量及血尿酸浓度。结果三组小鼠治疗前后组间、组内体质量比较,差异均无统计学意义(P>0.05)。时间与方法在足掌厚度增长百分比上存在交互作用(P<0.001);时间、方法在足掌厚度增长百分比上主效应显著(P<0.001)。溶剂组和DGNTT组小鼠造模后6 h及治疗后1~7 d足掌厚度增长百分比大于对照组,DGNTT组小鼠造模后6 h及治疗后1~5 d、7 d足掌厚度增长百分比小于溶剂组(P<0.05)。溶剂组和DGNTT组小鼠治疗后7 d注射足部组织IL-1β、TNF-α、iNOS mRNA相对表达量高于对照组,DGNTT组小鼠治疗后7 d注射足部组织IL-1β、TNF-α、iNOS mRNA相对表达量低于溶剂组(P<0.01)。DGNTT组小鼠治疗后7 d血尿酸浓度低于对照组、溶剂组(P<0.01)。结论当归拈痛汤可有效缓解AGA小鼠足爪炎性肿胀程度,下调炎性因子IL-1β、TNF-α、iNOS mRNA的表达,降低血尿酸浓度,是能够解决AGA多个核心问题的有效方剂且安全性较高。Background Traditional Chinese Medicine(TCM)compound preparations can lower the uric acid and relieve symptoms of acute gouty arthritis(AGA),a disease caused by multiple factors,through exerting multi-target effects of multiple ingredients,but the specific mechanism of action is not completely clear.Objective To investigate the efficacy and mechanism of action of Danggui Niantong Decoction(DND)against AGA.Methods This experiment was conducted from 17 August to 7 October 2020.After 1 week of adaptive feeding,30 numbered male SPF C57BL/6 mice aged 10-12 weeks were equally randomized into control,solvent and DND groups,receiving phosphate buffer,3%suspension of monosodium urate,and 3%suspension of monosodium urate injected at the area around deep tarsometatarsal joints of both hind paws,respectively,for modeling.After modeling,control and solvent groups received intragastric administration of 0.9%sodium chloride injection for 1 week,while DND group received intragastric administration of DND solution for 1 week.Comparison was conducted in the three groups,involving body weight before and after treatment,increased percentage of hind paws thickness 6 hours after modeling and 1 to 7 days after treatment,relative mRNA expression quantities of IL-1β,TNF-αand iNOS in the injected hind paw tissues and serum uric acid concentration 7 days after treatment.Results No significant inter-or intra-group difference in body weight was found in the three groups before or after treatment(P>0.05).There was significant interaction in the increased percentage of hind paws thickness between intervention time and method(P<0.001).The main effects of intervention time and method were both significant in the increased percentage of hind paws thickness(P<0.001).Increased percentage of hind paws thickness in solvent group or DND group was thicker than that in control group 6 hours after modeling and 1 to 7 days after treatment,so was in DND group compared to solvent group except for the time point of the 6th day after treatment(P<0.05).The c

关 键 词：关节炎 痛风性 当归拈痛散 当归拈痛汤 治疗结果 动物实验 小鼠 近交C57BL 

分 类 号：R589.7[医药卫生—内分泌]